TY - JOUR
T1 - The effect of molybdenum on the molecular
control of cold tolerance in cauliflower
(Brassica oleracea var. botrytis) artificial
seeds
AU - Rihan, Hail Z.
AU - Al-Issawi, Mohammed
AU - Woldie, Wondwossen Abate
AU - Kiernan, Michele
AU - Fuller, Michael P.
PY - 2014/7/7
Y1 - 2014/7/7
N2 - Molybdenum (Mo) was used to improve the
cold tolerance of cauliflower microshoots/artificial seeds.
The optimal stage to introduce Mo to the micropropagation
system was found to be the microshoots liquid culture
stage, since its use within artificial seed capsules or conversion
media had negative effects on conversion rate and
viability. Mo was found to improve the cold tolerance of
both acclimated and non-acclimated microshoots/artificial
seeds. The capacity of Mo to up-regulate CBF/DREB1 in
cauliflower microshoots was confirmed. Moreover, this
study is the first to confirm the ability of this element to upregulate
CBF/DREB1 without any low temperature treatment.
Mo significantly increased the accumulation of
23 kDa polypeptide when it was used at 15 ppm concentration.
However, Mo had a negative effect on the accumulation
of dehydrin proteins which suggest that this group
of proteins have no significant role in the cold tolerance of
cauliflower microshoots. The study could help in
improving the understanding of the abiotic stress network
in plants and in improving the quality and efficiency of
cauliflower artificial seed production systems.
AB - Molybdenum (Mo) was used to improve the
cold tolerance of cauliflower microshoots/artificial seeds.
The optimal stage to introduce Mo to the micropropagation
system was found to be the microshoots liquid culture
stage, since its use within artificial seed capsules or conversion
media had negative effects on conversion rate and
viability. Mo was found to improve the cold tolerance of
both acclimated and non-acclimated microshoots/artificial
seeds. The capacity of Mo to up-regulate CBF/DREB1 in
cauliflower microshoots was confirmed. Moreover, this
study is the first to confirm the ability of this element to upregulate
CBF/DREB1 without any low temperature treatment.
Mo significantly increased the accumulation of
23 kDa polypeptide when it was used at 15 ppm concentration.
However, Mo had a negative effect on the accumulation
of dehydrin proteins which suggest that this group
of proteins have no significant role in the cold tolerance of
cauliflower microshoots. The study could help in
improving the understanding of the abiotic stress network
in plants and in improving the quality and efficiency of
cauliflower artificial seed production systems.
U2 - 10.1007/s11240-014-0475-7
DO - 10.1007/s11240-014-0475-7
M3 - Article
SN - 1573-5044
VL - 0
SP - 215
EP - 228
JO - Plant, Cell, Tissue, Organ Culture (PCTOC) Journal of Plant Biotechnology
JF - Plant, Cell, Tissue, Organ Culture (PCTOC) Journal of Plant Biotechnology
IS - 2
ER -