Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia

B. B. Rees; T. E. Targett; B. J. Ciotti; C. A. Tolman; S. S. Akkina; A. M. Gallaty

doi:10.1111/j.1095-8649.2012.03319.x

Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia

B. B. Rees^*, T. E. Targett, B. J. Ciotti, C. A. Tolman, S. S. Akkina, A. M. Gallaty

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l−1), moderate hypoxia (3·0 mg O2 l−1), normoxia (7·1 mg O2 l−1) and hyperoxia (10·6 mg O2 l−1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two‐week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.

Original language	English
Pages (from-to)	148-164
Number of pages	0
Journal	Journal of Fish Biology
Volume	81
Issue number	1
Early online date	22 May 2012
DOIs	https://doi.org/10.1111/j.1095-8649.2012.03319.x
Publication status	Published - Jul 2012

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title = "Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia",

abstract = "Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l−1), moderate hypoxia (3·0 mg O2 l−1), normoxia (7·1 mg O2 l−1) and hyperoxia (10·6 mg O2 l−1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two‐week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.",

author = "Rees, {B. B.} and Targett, {T. E.} and Ciotti, {B. J.} and Tolman, {C. A.} and Akkina, {S. S.} and Gallaty, {A. M.}",

year = "2012",

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doi = "10.1111/j.1095-8649.2012.03319.x",

language = "English",

volume = "81",

pages = "148--164",

journal = "Journal of Fish Biology",

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T1 - Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia

AU - Rees, B. B.

AU - Targett, T. E.

AU - Ciotti, B. J.

AU - Tolman, C. A.

AU - Akkina, S. S.

AU - Gallaty, A. M.

PY - 2012/7

Y1 - 2012/7

N2 - Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l−1), moderate hypoxia (3·0 mg O2 l−1), normoxia (7·1 mg O2 l−1) and hyperoxia (10·6 mg O2 l−1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two‐week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.

AB - Specific growth rate (GS) and white skeletal muscle composition were measured in the mummichog Fundulus heteroclitus over a period of 28 days at four levels of dissolved oxygen (DO): severe hypoxia (c. 1·2 mg O2 l−1), moderate hypoxia (3·0 mg O2 l−1), normoxia (7·1 mg O2 l−1) and hyperoxia (10·6 mg O2 l−1). The GS was calculated over 0–8, 0–14, 0–28 and 14–28 days, and muscle protein, lactate dehydrogenase (LDH), DNA, RNA and water were measured at 0, 8, 14 and 28 days. Exposure of fish to severe hypoxia was associated with significantly reduced GS, lower muscle protein content and lower RNA:DNA compared with other DO treatments. When calculated over the first and second half of the 28 day exposure, however, GS of fish in severe hypoxia increased significantly during the second two‐week interval, to the same rate as that of normoxic fish. Muscle LDH activity and water content were not significantly affected by DO level. Neither moderate hypoxia nor hyperoxia significantly affected GS or any biochemical variable. The results demonstrate that F. heteroclitus can tolerate wide variation in ambient oxygen concentration and, during prolonged exposure to severe hypoxia, shows significant compensation for the initial negative effects on growth. The capacity of F. heteroclitus to grow over a wide range of DO probably contributes to its ability to exploit habitats characterized by marked variation in oxygen availability.

U2 - 10.1111/j.1095-8649.2012.03319.x

DO - 10.1111/j.1095-8649.2012.03319.x

M3 - Article

SN - 0022-1112

VL - 81

SP - 148

EP - 164

JO - Journal of Fish Biology

JF - Journal of Fish Biology

IS - 1

ER -

Temporal dynamics in growth and white skeletal muscle composition of the mummichog Fundulus heteroclitus during chronic hypoxia and hyperoxia

Abstract

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