Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division

Anthony G. Uren; Traude Beilharz; MJ O’Connell; Sarah J. Bugg; Driel R van; David L. Vaux; Trevor Lithgow

doi:10.1073/pnas.96.18.10170

Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division

Anthony G. Uren, Traude Beilharz, MJ O’Connell, Sarah J. Bugg, Driel R van, David L. Vaux^*, Trevor Lithgow

^*Corresponding author for this work

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster . The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both encode a single IAP, designated BIR1 and bir1 , respectively, each of which bears two BIRs. In rich medium, BIR1 mutant S. cerevisiae underwent normal vegetative growth and mitosis. Under starvation conditions, however, BIR1 mutant diploids formed spores inefficiently, instead undergoing pseudohyphal differentiation. Most spores that did form failed to survive beyond two divisions after germination. bir1 mutant S. pombe spores also died in the early divisions after spore germination and became blocked at the metaphase/anaphase transition because of an inability to elongate their mitotic spindle. Rather than inhibiting caspase-mediated cell death, yeast IAP proteins have roles in cell division and appear to act in a similar way to the IAPs from Caenorhabditis elegans and the mammalian IAP Survivin.

Original language	English
Pages (from-to)	10170-10175
Number of pages	0
Journal	Proceedings of the National Academy of Sciences
Volume	96
Issue number	18
DOIs	https://doi.org/10.1073/pnas.96.18.10170
Publication status	Published - 31 Aug 1999

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title = "Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division",

abstract = " Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster . The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both encode a single IAP, designated BIR1 and bir1 , respectively, each of which bears two BIRs. In rich medium, BIR1 mutant S. cerevisiae underwent normal vegetative growth and mitosis. Under starvation conditions, however, BIR1 mutant diploids formed spores inefficiently, instead undergoing pseudohyphal differentiation. Most spores that did form failed to survive beyond two divisions after germination. bir1 mutant S. pombe spores also died in the early divisions after spore germination and became blocked at the metaphase/anaphase transition because of an inability to elongate their mitotic spindle. Rather than inhibiting caspase-mediated cell death, yeast IAP proteins have roles in cell division and appear to act in a similar way to the IAPs from Caenorhabditis elegans and the mammalian IAP Survivin. ",

author = "Uren, {Anthony G.} and Traude Beilharz and MJ O{\textquoteright}Connell and Bugg, {Sarah J.} and van, {Driel R} and Vaux, {David L.} and Trevor Lithgow",

year = "1999",

month = aug,

day = "31",

doi = "10.1073/pnas.96.18.10170",

language = "English",

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journal = "Proceedings of the National Academy of Sciences",

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T1 - Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division

AU - Uren, Anthony G.

AU - Beilharz, Traude

AU - O’Connell, MJ

AU - Bugg, Sarah J.

AU - van, Driel R

AU - Vaux, David L.

AU - Lithgow, Trevor

PY - 1999/8/31

Y1 - 1999/8/31

N2 - Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster . The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both encode a single IAP, designated BIR1 and bir1 , respectively, each of which bears two BIRs. In rich medium, BIR1 mutant S. cerevisiae underwent normal vegetative growth and mitosis. Under starvation conditions, however, BIR1 mutant diploids formed spores inefficiently, instead undergoing pseudohyphal differentiation. Most spores that did form failed to survive beyond two divisions after germination. bir1 mutant S. pombe spores also died in the early divisions after spore germination and became blocked at the metaphase/anaphase transition because of an inability to elongate their mitotic spindle. Rather than inhibiting caspase-mediated cell death, yeast IAP proteins have roles in cell division and appear to act in a similar way to the IAPs from Caenorhabditis elegans and the mammalian IAP Survivin.

AB - Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster . The yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe both encode a single IAP, designated BIR1 and bir1 , respectively, each of which bears two BIRs. In rich medium, BIR1 mutant S. cerevisiae underwent normal vegetative growth and mitosis. Under starvation conditions, however, BIR1 mutant diploids formed spores inefficiently, instead undergoing pseudohyphal differentiation. Most spores that did form failed to survive beyond two divisions after germination. bir1 mutant S. pombe spores also died in the early divisions after spore germination and became blocked at the metaphase/anaphase transition because of an inability to elongate their mitotic spindle. Rather than inhibiting caspase-mediated cell death, yeast IAP proteins have roles in cell division and appear to act in a similar way to the IAPs from Caenorhabditis elegans and the mammalian IAP Survivin.

U2 - 10.1073/pnas.96.18.10170

DO - 10.1073/pnas.96.18.10170

M3 - Article

SN - 0027-8424

VL - 96

SP - 10170

EP - 10175

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

IS - 18

ER -

Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division

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