Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

Costa D Da; Marine Turek; Daniel J. Felmlee; Erika Girardi; Sébastien Pfeffer; Gang Long; Ralf Bartenschlager; Mirjam B. Zeisel; Thomas F. Baumert

doi:10.1128/jvi.01066-12

Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

Costa D Da, Marine Turek, Daniel J. Felmlee, Erika Girardi, Sébastien Pfeffer, Gang Long, Ralf Bartenschlager, Mirjam B. Zeisel^*, Thomas F. Baumert

^*Corresponding author for this work

Peninsula Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

ABSTRACT Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

Original language	English
Pages (from-to)	11919-11925
Number of pages	0
Journal	Journal of Virology
Volume	86
Issue number	21
DOIs	https://doi.org/10.1128/jvi.01066-12
Publication status	Published - Nov 2012

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10.1128/jvi.01066-12

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title = "Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells",

abstract = "ABSTRACT Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.",

author = "Da, {Costa D} and Marine Turek and Felmlee, {Daniel J.} and Erika Girardi and S{\'e}bastien Pfeffer and Gang Long and Ralf Bartenschlager and Zeisel, {Mirjam B.} and Baumert, {Thomas F.}",

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T1 - Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

AU - Da, Costa D

AU - Turek, Marine

AU - Felmlee, Daniel J.

AU - Girardi, Erika

AU - Pfeffer, Sébastien

AU - Long, Gang

AU - Bartenschlager, Ralf

AU - Zeisel, Mirjam B.

AU - Baumert, Thomas F.

PY - 2012/11

Y1 - 2012/11

N2 - ABSTRACT Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

AB - ABSTRACT Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

U2 - 10.1128/jvi.01066-12

DO - 10.1128/jvi.01066-12

M3 - Article

SN - 0022-538X

VL - 86

SP - 11919

EP - 11925

JO - Journal of Virology

JF - Journal of Virology

IS - 21

ER -

Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

Abstract

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