Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).

Richard A. Billington; Jan W. Thuring; Stuart J. Conway; Len Packman; Andrew B. Holmes; Armando A. Genazzani

doi:10.1042/BJ20031284

Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).

Richard A. Billington, Jan W. Thuring, Stuart J. Conway, Len Packman, Andrew B. Holmes, Armando A. Genazzani^*

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca2+-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor, the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

Original language	English
Pages (from-to)	275-280
Number of pages	0
Journal	Biochem J
Volume	378
Issue number	0
DOIs	https://doi.org/10.1042/BJ20031284
Publication status	Published - 15 Feb 2004

Keywords

Animals
Calcium
Carrier Proteins
Glucosephosphate Dehydrogenase
NADP
Ovum
Oxidation-Reduction
Sea Urchins

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title = "Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).",

abstract = "The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca2+-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor, the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.",

keywords = "Animals, Calcium, Carrier Proteins, Glucosephosphate Dehydrogenase, NADP, Ovum, Oxidation-Reduction, Sea Urchins",

author = "Billington, {Richard A.} and Thuring, {Jan W.} and Conway, {Stuart J.} and Len Packman and Holmes, {Andrew B.} and Genazzani, {Armando A.}",

year = "2004",

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doi = "10.1042/BJ20031284",

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T1 - Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).

AU - Billington, Richard A.

AU - Thuring, Jan W.

AU - Conway, Stuart J.

AU - Packman, Len

AU - Holmes, Andrew B.

AU - Genazzani, Armando A.

PY - 2004/2/15

Y1 - 2004/2/15

N2 - The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca2+-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor, the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

AB - The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca2+-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor, the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

KW - Animals

KW - Calcium

KW - Carrier Proteins

KW - Glucosephosphate Dehydrogenase

KW - NADP

KW - Ovum

KW - Oxidation-Reduction

KW - Sea Urchins

U2 - 10.1042/BJ20031284

DO - 10.1042/BJ20031284

M3 - Article

SN - 1470-8728

VL - 378

SP - 275

EP - 280

JO - Biochem J

JF - Biochem J

IS - 0

ER -

Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).

Abstract

Keywords

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