Polymorphic differences in SOD-2 may influence HCV viral clearance.

Annwyne Houldsworth*, Magdalena Metzner, Steve Shaw, Edward Kaminski, Andy G. Demaine, Matthew E. Cramp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatitis C virus (HCV) is a pathogen causing chronic hepatitis, cirrhosis, and liver cancer occurring in about 3% of the world's population. Most individuals infected with HCV develop persistent viremia. Oxidative stress may play an important role in the pathogenesis of a number of diseases including HCV infection and diabetes mellitus. Polymorphisms in the antioxidant genes may determine cellular oxidative stress levels as a primary pathogenic role in HCV and/or in its complications. Patients with HCV and normal, healthy controls were investigated for a superoxide dismutase (SOD-2) polymorphism in the mitochondrial targeting sequence with Ala/Val (C-9T) substitution. Polymorphisms in antioxidant gene SOD-2 were carried out by PCR, restriction fragment length polymorphism assays and by polyacrylamide gel electrophoresis. For the SOD-2 polymorphism, the RNA positive group showed a higher percentage of "CT" genotype than the RNA negative group (89.3% vs. 66.1%, P = 0.001, χ(2)  = 11.9). The RNA negative group had more TT genotypes than the RNA positive group (27.4% vs. 6.80%, P = 0.01, χ(2)  = 11.6). The exposed uninfected group had an increased frequency of the "CT" genotype (86.2% vs. 66.1%, P = 0.02, χ(2)  = 5.5). The RNA positives had a higher frequency of the "CT" from the normal controls (72.1% vs. 89.2%, P = 0.005, χ(2)  = 7.8).
Original languageEnglish
Pages (from-to)941-947
Number of pages0
JournalJ Med Virol
Volume86
Issue number6
DOIs
Publication statusPublished - Jun 2014

Keywords

  • SOD-2
  • antioxidant
  • gene polymorphism
  • hepatitis C
  • virus clearance
  • Adult
  • Electrophoresis
  • Polyacrylamide Gel
  • Female
  • Genetic Predisposition to Disease
  • Hepacivirus
  • Hepatitis C
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism
  • Restriction Fragment Length
  • Single Nucleotide
  • Retrospective Studies
  • Superoxide Dismutase

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