Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.

L Godfrey; I Bailey; NJ Toms; GD Clarke; I Kitchen; SMO Hourani

doi:10.1016/j.ejphar.2007.01.075

Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.

L Godfrey, I Bailey, NJ Toms, GD Clarke, I Kitchen, SMO Hourani

Peninsula Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.

Original language	English
Pages (from-to)	68-71
Number of pages	0
Journal	Eur J Pharmacol
Volume	562
Issue number	0
DOIs	https://doi.org/10.1016/j.ejphar.2007.01.075
Publication status	Published - 7 May 2007

Keywords

Acetaminophen
Analgesics
Non-Narcotic
Animals
Arginine
Caffeine
Central Nervous System Stimulants
Cerebellum
Citrulline
Dose-Response Relationship
Drug
Enzyme Inhibitors
Glutamic Acid
In Vitro Techniques
Male
Mice
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Spinal Cord

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10.1016/j.ejphar.2007.01.075

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title = "Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.",

abstract = "Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.",

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author = "L Godfrey and I Bailey and NJ Toms and GD Clarke and I Kitchen and SMO Hourani",

year = "2007",

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doi = "10.1016/j.ejphar.2007.01.075",

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T1 - Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.

AU - Godfrey, L

AU - Bailey, I

AU - Toms, NJ

AU - Clarke, GD

AU - Kitchen, I

AU - Hourani, SMO

PY - 2007/5/7

Y1 - 2007/5/7

N2 - Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.

AB - Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [(3)H]arginine to [(3)H]citrulline. Paracetamol (100 microM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 microM) and caffeine (30 microM) attenuated glutamate (5 mM)-induced [(3)H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.

KW - Acetaminophen

KW - Analgesics

KW - Non-Narcotic

KW - Animals

KW - Arginine

KW - Caffeine

KW - Central Nervous System Stimulants

KW - Cerebellum

KW - Citrulline

KW - Dose-Response Relationship

KW - Drug

KW - Enzyme Inhibitors

KW - Glutamic Acid

KW - In Vitro Techniques

KW - Male

KW - Mice

KW - NG-Nitroarginine Methyl Ester

KW - Nitric Oxide

KW - Nitric Oxide Synthase

KW - Spinal Cord

U2 - 10.1016/j.ejphar.2007.01.075

DO - 10.1016/j.ejphar.2007.01.075

M3 - Article

SN - 0014-2999

VL - 562

SP - 68

EP - 71

JO - Eur J Pharmacol

JF - Eur J Pharmacol

IS - 0

ER -

Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices.

Abstract

Keywords

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