Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni

Waheed Jowiya; Katja Brunner; Sherif Abouelhadid; Haitham A. Hussain; Sean P. Nair; Sohaib Sadiq; Lisa K. Williams; Emma K. Trantham; Holly Stephenson; Brendan W. Wren; Mona Bajaj-Elliott; Tristan A. Cogan; Andrew P. Laws; Jim Wade; Nick Dorrell; Elaine Allan

doi:10.1128/iai.01064-15

Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni

Waheed Jowiya
, Katja Brunner
, Sherif Abouelhadid
, Haitham A. Hussain
, Sean P. Nair
, Sohaib Sadiq
, Lisa K. Williams
, Emma K. Trantham
, Holly Stephenson
, Brendan W. Wren
, Mona Bajaj-Elliott
, Tristan A. Cogan
, Andrew P. Laws
, Jim Wade
, Nick Dorrell
, Elaine Allan^*

^*Corresponding author for this work

Peninsula Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

ABSTRACT Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro , increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro , suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.

Original language	English
Pages (from-to)	4884-4895
Number of pages	0
Journal	Infection and Immunity
Volume	83
Issue number	12
DOIs	https://doi.org/10.1128/iai.01064-15
Publication status	Published - Dec 2015

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Jowiya, W., Brunner, K., Abouelhadid, S., Hussain, H. A., Nair, S. P., Sadiq, S., Williams, L. K., Trantham, E. K., Stephenson, H., Wren, B. W., Bajaj-Elliott, M., Cogan, T. A., Laws, A. P., Wade, J., Dorrell, N., & Allan, E. (2015). Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni. Infection and Immunity, 83(12), 4884-4895. https://doi.org/10.1128/iai.01064-15

@article{bb5fbf14d93b44279719327748a1bbba,

title = "Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni",

abstract = "ABSTRACT Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro , increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro , suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism. ",

author = "Waheed Jowiya and Katja Brunner and Sherif Abouelhadid and Hussain, \{Haitham A.\} and Nair, \{Sean P.\} and Sohaib Sadiq and Williams, \{Lisa K.\} and Trantham, \{Emma K.\} and Holly Stephenson and Wren, \{Brendan W.\} and Mona Bajaj-Elliott and Cogan, \{Tristan A.\} and Laws, \{Andrew P.\} and Jim Wade and Nick Dorrell and Elaine Allan",

year = "2015",

month = dec,

doi = "10.1128/iai.01064-15",

language = "English",

volume = "83",

pages = "4884--4895",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

number = "12",

}

Jowiya, W, Brunner, K, Abouelhadid, S, Hussain, HA, Nair, SP, Sadiq, S, Williams, LK, Trantham, EK, Stephenson, H, Wren, BW, Bajaj-Elliott, M, Cogan, TA, Laws, AP, Wade, J, Dorrell, N & Allan, E 2015, 'Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni', Infection and Immunity, vol. 83, no. 12, pp. 4884-4895. https://doi.org/10.1128/iai.01064-15

TY - JOUR

T1 - Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni

AU - Jowiya, Waheed

AU - Brunner, Katja

AU - Abouelhadid, Sherif

AU - Hussain, Haitham A.

AU - Nair, Sean P.

AU - Sadiq, Sohaib

AU - Williams, Lisa K.

AU - Trantham, Emma K.

AU - Stephenson, Holly

AU - Wren, Brendan W.

AU - Bajaj-Elliott, Mona

AU - Cogan, Tristan A.

AU - Laws, Andrew P.

AU - Wade, Jim

AU - Dorrell, Nick

AU - Allan, Elaine

PY - 2015/12

Y1 - 2015/12

N2 - ABSTRACT Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro , increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro , suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.

AB - ABSTRACT Campylobacter jejuni is a commensal bacterium in the intestines of animals and birds and a major cause of food-borne gastroenteritis in humans worldwide. Here we show that exposure to pancreatic amylase leads to secretion of an α-dextran by C. jejuni and that a secreted protease, Cj0511, is required. Exposure of C. jejuni to pancreatic amylase promotes biofilm formation in vitro , increases interaction with human epithelial cell lines, increases virulence in the Galleria mellonella infection model, and promotes colonization of the chicken ileum. We also show that exposure to pancreatic amylase protects C. jejuni from stress conditions in vitro , suggesting that the induced α-dextran may be important during transmission between hosts. This is the first evidence that pancreatic amylase functions as an interkingdom signal in an enteric microorganism.

U2 - 10.1128/iai.01064-15

DO - 10.1128/iai.01064-15

M3 - Article

SN - 0019-9567

VL - 83

SP - 4884

EP - 4895

JO - Infection and Immunity

JF - Infection and Immunity

IS - 12

ER -

Pancreatic Amylase Is an Environmental Signal for Regulation of Biofilm Formation and Host Interaction in Campylobacter jejuni

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