Abstract
Abstract
Background: Using volatile organic compounds (VOCs) may be a suitable alternative to invasive tests for diagnosing and phenotyping childhood asthma. The ReCIVA (Owlstone Medical Limited) breath sampler is designed to standardise breath sample collection for offline analysis of VOCs. Untargeted VOC analysis may be affected by batch effects.
Aim: To determine whether VOCs obtained by the ReCIVA breath sampler in asthmatic children are subject to batch effects.
Methods: 90 ReCIVA breath samples were collected from 64 children (5-15 years) with and without asthma. Batch effect analysis was completed for 8 operational features including age, gender, breathing rate, sample collection time, ReCIVA serial number, season, time of day and ReCIVA operator. The Wilcoxon rank sum test and Kruskal-Wallis test were used to test for a difference in both row means and variances for each batch effect.
Results: 70 (77.8%) samples of 90 collected the 1L target breath volume. 43 samples were available for batch effect analysis, for which untargeted VOC analysis indicated no significant batch effects for all 8 operational factors investigated (Figure 1 shows row means across gender with (1) female and (2) male).
Background: Using volatile organic compounds (VOCs) may be a suitable alternative to invasive tests for diagnosing and phenotyping childhood asthma. The ReCIVA (Owlstone Medical Limited) breath sampler is designed to standardise breath sample collection for offline analysis of VOCs. Untargeted VOC analysis may be affected by batch effects.
Aim: To determine whether VOCs obtained by the ReCIVA breath sampler in asthmatic children are subject to batch effects.
Methods: 90 ReCIVA breath samples were collected from 64 children (5-15 years) with and without asthma. Batch effect analysis was completed for 8 operational features including age, gender, breathing rate, sample collection time, ReCIVA serial number, season, time of day and ReCIVA operator. The Wilcoxon rank sum test and Kruskal-Wallis test were used to test for a difference in both row means and variances for each batch effect.
Results: 70 (77.8%) samples of 90 collected the 1L target breath volume. 43 samples were available for batch effect analysis, for which untargeted VOC analysis indicated no significant batch effects for all 8 operational factors investigated (Figure 1 shows row means across gender with (1) female and (2) male).
| Original language | English |
|---|---|
| Article number | 2761 |
| Journal | European Respiratory Journal |
| Volume | 60 |
| Issue number | Supp 66 |
| DOIs | |
| Publication status | Published - 4 Sept 2022 |
| Event | ERS International Congress 2022 abstracts - Duration: 4 Sept 2022 → … |