Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia.

AD Alanazi, Robert Puschendorf, Bashir Salim, Mohamed S. Alyousif, IO Alanazi, Hajri R. Al-shehri

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Abstract

We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species-specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.
Original languageEnglish
Number of pages0
JournalJournal of Veterinary Diagnostic Investigation
Volume30
Issue number6
DOIs
Publication statusPublished - Nov 2018

Keywords

  • Equine
  • ITS1
  • PCR
  • RoTat1.2
  • Saudi Arabia
  • trypanosomiasis

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