Measurement of Proton Leak in Isolated Mitochondria.

Charles Affourtit; Hoi Shan Wong; Martin D. Brand

doi:10.1007/978-1-4939-7831-1_9

Measurement of Proton Leak in Isolated Mitochondria.

Charles Affourtit, Hoi Shan Wong, Martin D. Brand^*

^*Corresponding author for this work

School of Biomedical Sciences

Buck Institute for Age Research

Research output: Contribution to journal › Article › peer-review

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Abstract

Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized.

Original language	English
Pages (from-to)	157-170
Number of pages	0
Journal	Methods in Molecular Biology
Volume	1782
Issue number	0
DOIs	https://doi.org/10.1007/978-1-4939-7831-1_9
Publication status	Published - 23 May 2018

Keywords

Membrane potential
Mitochondria
Modular kinetic analysis
Oxidative phosphorylation
Oxygen consumption

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10.1007/978-1-4939-7831-1_9

Affourtit et al 2018 Mitochondrial proton leak

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abstract = "Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized.",

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AU - Wong, Hoi Shan

AU - Brand, Martin D.

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AB - Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized.

KW - Membrane potential

KW - Mitochondria

KW - Modular kinetic analysis

KW - Oxidative phosphorylation

KW - Oxygen consumption

U2 - 10.1007/978-1-4939-7831-1_9

DO - 10.1007/978-1-4939-7831-1_9

M3 - Article

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SP - 157

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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Measurement of Proton Leak in Isolated Mitochondria.

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Keywords

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