Measurement of proton leak and electron leak in isolated mitochondria.

Charles Affourtit; Casey L. Quinlan; Martin D. Brand

doi:10.1007/978-1-61779-382-0_11

Measurement of proton leak and electron leak in isolated mitochondria.

Charles Affourtit, Casey L. Quinlan, Martin D. Brand^*

^*Corresponding author for this work

School of Biomedical Sciences

Buck Institute for Age Research

Research output: Contribution to journal › Article › peer-review

Abstract

Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.

Original language	English
Pages (from-to)	165-182
Number of pages	0
Journal	Methods Mol Biol
Volume	810
Issue number	0
DOIs	https://doi.org/10.1007/978-1-61779-382-0_11
Publication status	Published - 2012

Keywords

Animals
Electrons
Kinetics
Membrane Potential
Mitochondrial
Mitochondria
Muscle
Oxidative Phosphorylation
Oxygen Consumption
Protons
Rats
Wistar

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10.1007/978-1-61779-382-0_11

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title = "Measurement of proton leak and electron leak in isolated mitochondria.",

abstract = "Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.",

keywords = "Animals, Electrons, Kinetics, Membrane Potential, Mitochondrial, Mitochondria, Muscle, Oxidative Phosphorylation, Oxygen Consumption, Protons, Rats, Wistar",

author = "Charles Affourtit and Quinlan, {Casey L.} and Brand, {Martin D.}",

year = "2012",

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AU - Affourtit, Charles

AU - Quinlan, Casey L.

AU - Brand, Martin D.

PY - 2012

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N2 - Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.

AB - Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton and electron leaks. In this chapter, methods are presented to measure proton and electron leak activities in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasised.

KW - Animals

KW - Electrons

KW - Kinetics

KW - Membrane Potential

KW - Mitochondrial

KW - Mitochondria

KW - Muscle

KW - Oxidative Phosphorylation

KW - Oxygen Consumption

KW - Protons

KW - Rats

KW - Wistar

U2 - 10.1007/978-1-61779-382-0_11

DO - 10.1007/978-1-61779-382-0_11

M3 - Article

SN - 1940-6029

VL - 810

SP - 165

EP - 182

JO - Methods Mol Biol

JF - Methods Mol Biol

IS - 0

ER -

Measurement of proton leak and electron leak in isolated mitochondria.

Abstract

Keywords

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