Abstract
Bradykinin and substance P have been derivatised with cyclic diethylenetriaminepentaacetic anhydride (cDTPA) and subsequently labelled with natural and isotopically enriched Eu(3+). This enabled the detection and relative quantitation of the peptides using element-selective detection by high-performance liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS). Relative quantitation was achieved by differentially labelling two peptide sources, after derivatisation with cDTPA, using natural and enriched (151)Eu respectively. The (151)Eu:(153)Eu isotope ratio was measured and used to calculate the original peptide ratio. The measured ratios came within 5.2% of the known ratio. Derivatisation and chelation reactions were additionally confirmed using LC-ESI-MS.
Original language | English |
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Pages (from-to) | 61-65 |
Number of pages | 0 |
Journal | Anal Bioanal Chem |
Volume | 390 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2008 |
Keywords
- Bradykinin
- Chromatography
- Liquid
- Europium
- Isotopes
- Mass Spectrometry
- Substance P