Isotopic labelling of peptides and isotope ratio analysis using LC-ICP-MS: a preliminary study.

P Patel, P Jones, R Handy, C Harrington, P Marshall, EH Evans

Research output: Contribution to journalArticlepeer-review

Abstract

Bradykinin and substance P have been derivatised with cyclic diethylenetriaminepentaacetic anhydride (cDTPA) and subsequently labelled with natural and isotopically enriched Eu(3+). This enabled the detection and relative quantitation of the peptides using element-selective detection by high-performance liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS). Relative quantitation was achieved by differentially labelling two peptide sources, after derivatisation with cDTPA, using natural and enriched (151)Eu respectively. The (151)Eu:(153)Eu isotope ratio was measured and used to calculate the original peptide ratio. The measured ratios came within 5.2% of the known ratio. Derivatisation and chelation reactions were additionally confirmed using LC-ESI-MS.
Original languageEnglish
Pages (from-to)61-65
Number of pages0
JournalAnal Bioanal Chem
Volume390
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Bradykinin
  • Chromatography
  • Liquid
  • Europium
  • Isotopes
  • Mass Spectrometry
  • Substance P

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