Is there Ca2+(Sr2+)-3-hydroxybutyrate symport in rat-liver mitochondria? A reappraisal.

AJ Moody, IC West, R Mitchell, P Mitchell

Research output: Contribution to journalArticlepeer-review

Abstract

The observation in this laboratory that respiration and Sr2+ import were stimulated by the addition of 3-hydroxybutyrate to suspensions of N-ethylmaleimide-treated mitochondria respiring in state 6, after the addition of Sr2+, in a sucrose medium containing choline as substrate, led to the proposal by Moyle and Mitchell [(1977) FEBS Lett. 84, 135-140] that there is a Ca2+(Sr2+)-3-hydroxybutyrate symporter in rat liver mitochondria. However, experiments described in the present paper support a different interpretation. Under the conditions of the experiments by Moyle and Mitchell, the rate of respiration and the poise of Sr2+ accumulation are mainly limited, not by delta mu H+, but by lack of respiratory substrate. Even though N-ethylmaleimide is a potent inhibitor of 3-hydroxybutyrate dehydrogenase, we have found that, somewhat surprisingly, under the special conditions of these experiments, sufficient 3-hydroxybutyrate dehydrogenase activity remains available to account for the 3-hydroxybutyrate-dependent respiratory stimulation and Sr2+ import.
Original languageEnglish
Pages (from-to)243-249
Number of pages0
JournalEur J Biochem
Volume157
Issue number2
DOIs
Publication statusPublished - 2 Jun 1986

Keywords

  • 3-Hydroxybutyric Acid
  • Acetoacetates
  • Animals
  • Biological Transport
  • Active
  • Butyrates
  • Butyric Acid
  • Calcimycin
  • Calcium
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • Ethylmaleimide
  • Hydrogen-Ion Concentration
  • Hydroquinones
  • Hydroxybutyrate Dehydrogenase
  • Hydroxybutyrates
  • Mitochondria
  • Liver
  • Oxygen Consumption
  • Rats
  • Rotenone
  • Strontium
  • Time Factors

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