Improved isotopic model based on 15N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N2O emissions in a clay grassland soil

A Castellano‐Hinojosa; N Loick; E Dixon; GP Matthews; D Lewicka‐Szczebak; R Well; R Bol; A Charteris; L Cardenas

doi:10.1002/rcm.8374

Improved isotopic model based on ¹⁵N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N₂O emissions in a clay grassland soil

A Castellano‐Hinojosa, N Loick, E Dixon, GP Matthews, D Lewicka‐Szczebak, R Well, R Bol, A Charteris, L Cardenas

School of Geography, Earth and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

RationaleIsotopic signatures of N2O can help distinguish between two sources (fertiliser N or endogenous soil N) of N2O emissions. The contribution of each source to N2O emissions after N‐application is difficult to determine. Here, isotopologue signatures of emitted N2O are used in an improved isotopic model based on Rayleigh‐type equations.MethodsThe effects of a partial (33% of surface area, treatment 1c) or total (100% of surface area, treatment 3c) dispersal of N and C on gaseous emissions from denitrification were measured in a laboratory incubation system (DENIS) allowing simultaneous measurements of NO, N2O, N2 and CO2 over a 12‐day incubation period. To determine the source of N2O emissions those results were combined with both the isotope ratio mass spectrometry analysis of the isotopocules of emitted N2O and those from the 15N‐tracing technique.ResultsThe spatial dispersal of N and C significantly affected the quantity, but not the timing, of gas fluxes. Cumulative emissions are larger for treatment 3c than treatment 1c. The 15N‐enrichment analysis shows that initially ~70% of the emitted N2O derived from the applied amendment followed by a constant decrease. The decrease in contribution of the fertiliser N‐pool after an initial increase is sooner and larger for treatment 1c. The Rayleigh‐type model applied to N2O isotopocules data (δ15Nbulk‐N2O values) shows poor agreement with the measurements for the original one‐pool model for treatment 1c; the two‐pool models gives better results when using a third‐order polynomial equation. In contrast, in treatment 3c little difference is observed between the two modelling approaches.ConclusionsThe importance of N2O emissions from different N‐pools in soil for the interpretation of N2O isotopocules data was demonstrated using a Rayleigh‐type model. Earlier statements concerning exponential increase in native soil nitrate pool activity highlighted in previous studies should be replaced with a polynomial increase with dependency on both N‐pool sizes.

Original language	English
Pages (from-to)	449-460
Number of pages	0
Journal	Rapid Communications in Mass Spectrometry
Volume	33
Issue number	5
Early online date	15 Feb 2019
DOIs	https://doi.org/10.1002/rcm.8374
Publication status	Published - 15 Mar 2019

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Castellano‐Hinojosa, A., Loick, N., Dixon, E., Matthews, GP., Lewicka‐Szczebak, D., Well, R., Bol, R., Charteris, A., & Cardenas, L. (2019). Improved isotopic model based on ¹⁵N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N₂O emissions in a clay grassland soil. Rapid Communications in Mass Spectrometry, 33(5), 449-460. https://doi.org/10.1002/rcm.8374

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title = "Improved isotopic model based on 15N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N2O emissions in a clay grassland soil",

abstract = "RationaleIsotopic signatures of N2O can help distinguish between two sources (fertiliser N or endogenous soil N) of N2O emissions. The contribution of each source to N2O emissions after N‐application is difficult to determine. Here, isotopologue signatures of emitted N2O are used in an improved isotopic model based on Rayleigh‐type equations.MethodsThe effects of a partial (33% of surface area, treatment 1c) or total (100% of surface area, treatment 3c) dispersal of N and C on gaseous emissions from denitrification were measured in a laboratory incubation system (DENIS) allowing simultaneous measurements of NO, N2O, N2 and CO2 over a 12‐day incubation period. To determine the source of N2O emissions those results were combined with both the isotope ratio mass spectrometry analysis of the isotopocules of emitted N2O and those from the 15N‐tracing technique.ResultsThe spatial dispersal of N and C significantly affected the quantity, but not the timing, of gas fluxes. Cumulative emissions are larger for treatment 3c than treatment 1c. The 15N‐enrichment analysis shows that initially ~70% of the emitted N2O derived from the applied amendment followed by a constant decrease. The decrease in contribution of the fertiliser N‐pool after an initial increase is sooner and larger for treatment 1c. The Rayleigh‐type model applied to N2O isotopocules data (δ15Nbulk‐N2O values) shows poor agreement with the measurements for the original one‐pool model for treatment 1c; the two‐pool models gives better results when using a third‐order polynomial equation. In contrast, in treatment 3c little difference is observed between the two modelling approaches.ConclusionsThe importance of N2O emissions from different N‐pools in soil for the interpretation of N2O isotopocules data was demonstrated using a Rayleigh‐type model. Earlier statements concerning exponential increase in native soil nitrate pool activity highlighted in previous studies should be replaced with a polynomial increase with dependency on both N‐pool sizes.",

author = "A Castellano‐Hinojosa and N Loick and E Dixon and GP Matthews and D Lewicka‐Szczebak and R Well and R Bol and A Charteris and L Cardenas",

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Castellano‐Hinojosa, A, Loick, N, Dixon, E, Matthews, GP, Lewicka‐Szczebak, D, Well, R, Bol, R, Charteris, A & Cardenas, L 2019, 'Improved isotopic model based on ¹⁵N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N₂O emissions in a clay grassland soil', Rapid Communications in Mass Spectrometry, vol. 33, no. 5, pp. 449-460. https://doi.org/10.1002/rcm.8374

Improved isotopic model based on ¹⁵N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N₂O emissions in a clay grassland soil. / Castellano‐Hinojosa, A; Loick, N; Dixon, E et al.
In: Rapid Communications in Mass Spectrometry, Vol. 33, No. 5, 15.03.2019, p. 449-460.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Improved isotopic model based on 15N tracing and Rayleigh‐type isotope fractionation for simulating differential sources of N2O emissions in a clay grassland soil

AU - Castellano‐Hinojosa, A

AU - Loick, N

AU - Dixon, E

AU - Matthews, GP

AU - Lewicka‐Szczebak, D

AU - Well, R

AU - Bol, R

AU - Charteris, A

AU - Cardenas, L

PY - 2019/3/15

Y1 - 2019/3/15

N2 - RationaleIsotopic signatures of N2O can help distinguish between two sources (fertiliser N or endogenous soil N) of N2O emissions. The contribution of each source to N2O emissions after N‐application is difficult to determine. Here, isotopologue signatures of emitted N2O are used in an improved isotopic model based on Rayleigh‐type equations.MethodsThe effects of a partial (33% of surface area, treatment 1c) or total (100% of surface area, treatment 3c) dispersal of N and C on gaseous emissions from denitrification were measured in a laboratory incubation system (DENIS) allowing simultaneous measurements of NO, N2O, N2 and CO2 over a 12‐day incubation period. To determine the source of N2O emissions those results were combined with both the isotope ratio mass spectrometry analysis of the isotopocules of emitted N2O and those from the 15N‐tracing technique.ResultsThe spatial dispersal of N and C significantly affected the quantity, but not the timing, of gas fluxes. Cumulative emissions are larger for treatment 3c than treatment 1c. The 15N‐enrichment analysis shows that initially ~70% of the emitted N2O derived from the applied amendment followed by a constant decrease. The decrease in contribution of the fertiliser N‐pool after an initial increase is sooner and larger for treatment 1c. The Rayleigh‐type model applied to N2O isotopocules data (δ15Nbulk‐N2O values) shows poor agreement with the measurements for the original one‐pool model for treatment 1c; the two‐pool models gives better results when using a third‐order polynomial equation. In contrast, in treatment 3c little difference is observed between the two modelling approaches.ConclusionsThe importance of N2O emissions from different N‐pools in soil for the interpretation of N2O isotopocules data was demonstrated using a Rayleigh‐type model. Earlier statements concerning exponential increase in native soil nitrate pool activity highlighted in previous studies should be replaced with a polynomial increase with dependency on both N‐pool sizes.

AB - RationaleIsotopic signatures of N2O can help distinguish between two sources (fertiliser N or endogenous soil N) of N2O emissions. The contribution of each source to N2O emissions after N‐application is difficult to determine. Here, isotopologue signatures of emitted N2O are used in an improved isotopic model based on Rayleigh‐type equations.MethodsThe effects of a partial (33% of surface area, treatment 1c) or total (100% of surface area, treatment 3c) dispersal of N and C on gaseous emissions from denitrification were measured in a laboratory incubation system (DENIS) allowing simultaneous measurements of NO, N2O, N2 and CO2 over a 12‐day incubation period. To determine the source of N2O emissions those results were combined with both the isotope ratio mass spectrometry analysis of the isotopocules of emitted N2O and those from the 15N‐tracing technique.ResultsThe spatial dispersal of N and C significantly affected the quantity, but not the timing, of gas fluxes. Cumulative emissions are larger for treatment 3c than treatment 1c. The 15N‐enrichment analysis shows that initially ~70% of the emitted N2O derived from the applied amendment followed by a constant decrease. The decrease in contribution of the fertiliser N‐pool after an initial increase is sooner and larger for treatment 1c. The Rayleigh‐type model applied to N2O isotopocules data (δ15Nbulk‐N2O values) shows poor agreement with the measurements for the original one‐pool model for treatment 1c; the two‐pool models gives better results when using a third‐order polynomial equation. In contrast, in treatment 3c little difference is observed between the two modelling approaches.ConclusionsThe importance of N2O emissions from different N‐pools in soil for the interpretation of N2O isotopocules data was demonstrated using a Rayleigh‐type model. Earlier statements concerning exponential increase in native soil nitrate pool activity highlighted in previous studies should be replaced with a polynomial increase with dependency on both N‐pool sizes.

U2 - 10.1002/rcm.8374

DO - 10.1002/rcm.8374

M3 - Article

SN - 0951-4198

VL - 33

SP - 449

EP - 460

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 5

ER -