Abstract
A high performance liquid chromatographic assay for the quantitative determination of apomorphine in human plasma is described. Sample clean-up and concentration was optimised using solid-phase extraction on C18 cartridges, enabling rapid and sensitive determination of apomorphine and potential metabolites. The limit of apomorphine quantification, using fluorescence detection, was 0.5 ng/mL. The assay was stability-indicating, and allowed the detection of analytes in the presence of commonly co-administered anti-Parkinsonian drugs. Apomorphine was stable in frozen plasma containing 0.14% (w/v) ascorbic acid for 98 days, and through four freeze-thaw cycles. The assay has been used in clinical pharmacokinetic studies of apomorphine in patients with Parkinson's disease, and in preliminary studies of novel apomorphine delivery devices in volunteers.
| Original language | English |
|---|---|
| Pages (from-to) | 1-7 |
| Number of pages | 0 |
| Journal | J Chromatogr B Analyt Technol Biomed Life Sci |
| Volume | 831 |
| Issue number | 0 |
| DOIs | |
| Publication status | Published - 2 Feb 2006 |
Keywords
- Apomorphine
- Ascorbic Acid
- Chromatography
- High Pressure Liquid
- Drug Stability
- Freezing
- Humans
- Parkinson Disease
- Reproducibility of Results
- Sensitivity and Specificity