TY - JOUR
T1 - Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.
AU - Dawson, S. E.
AU - Gibreel, T.
AU - Nicolaou, N.
AU - AlRabiah, H.
AU - Xu, Y.
AU - Goodacre, R.
AU - Upton, M.
PY - 2014/6
Y1 - 2014/6
N2 - In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.
AB - In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.
KW - Bacterial Typing Techniques
KW - Humans
KW - Spectroscopy
KW - Fourier Transform Infrared
KW - Uropathogenic Escherichia coli
UR - https://pearl.plymouth.ac.uk/context/bhs-research/article/1110/viewcontent/Dawson_EurJClinMicrobiolInfectDis2014.pdf
U2 - 10.1007/s10096-013-2036-0
DO - 10.1007/s10096-013-2036-0
M3 - Article
SN - 1435-4373
VL - 33
SP - 983
EP - 988
JO - Eur J Clin Microbiol Infect Dis
JF - Eur J Clin Microbiol Infect Dis
IS - 6
ER -