Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.

S. E. Dawson; T. Gibreel; N. Nicolaou; H. AlRabiah; Y. Xu; R. Goodacre; M. Upton

doi:10.1007/s10096-013-2036-0

Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.

S. E. Dawson, T. Gibreel, N. Nicolaou, H. AlRabiah, Y. Xu, R. Goodacre, M. Upton^*

^*Corresponding author for this work

School of Biomedical Sciences

University of Manchester

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Abstract

In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.

Original language	English
Pages (from-to)	983-988
Number of pages	0
Journal	Eur J Clin Microbiol Infect Dis
Volume	33
Issue number	6
DOIs	https://doi.org/10.1007/s10096-013-2036-0
Publication status	Published - Jun 2014

Keywords

Bacterial Typing Techniques
Humans
Spectroscopy
Fourier Transform Infrared
Uropathogenic Escherichia coli

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10.1007/s10096-013-2036-0

Dawson-EurJClinMicrobiolInfectDis2014

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title = "Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.",

abstract = "In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.",

keywords = "Bacterial Typing Techniques, Humans, Spectroscopy, Fourier Transform Infrared, Uropathogenic Escherichia coli",

author = "Dawson, {S. E.} and T. Gibreel and N. Nicolaou and H. AlRabiah and Y. Xu and R. Goodacre and M. Upton",

year = "2014",

month = jun,

doi = "10.1007/s10096-013-2036-0",

language = "English",

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T1 - Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.

AU - Dawson, S. E.

AU - Gibreel, T.

AU - Nicolaou, N.

AU - AlRabiah, H.

AU - Xu, Y.

AU - Goodacre, R.

AU - Upton, M.

PY - 2014/6

Y1 - 2014/6

N2 - In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.

AB - In this paper, we demonstrate that Fourier transform infrared (FT-IR) spectroscopy is able to discriminate rapidly between uropathogenic Escherichia coli (UPEC) of key lineages with only relatively simple sample preparation. A total of 95 bacteria from six different epidemiologically important multilocus sequence types (ST10, ST69, ST95, ST73, ST127 and ST131) were used in this project and principal component-discriminant function analysis (PC-DFA) of these samples produced clear separate clustering of isolates, based on the ST. Analysis of data using partial least squares-discriminant analysis (PLS-DA), incorporating cross-validation, indicated a high prediction accuracy of 91.19% for ST131. These results suggest that FT-IR spectroscopy could be a useful method for the rapid identification of members of important UPEC STs.

KW - Bacterial Typing Techniques

KW - Humans

KW - Spectroscopy

KW - Fourier Transform Infrared

KW - Uropathogenic Escherichia coli

U2 - 10.1007/s10096-013-2036-0

DO - 10.1007/s10096-013-2036-0

M3 - Article

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JO - Eur J Clin Microbiol Infect Dis

JF - Eur J Clin Microbiol Infect Dis

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ER -

Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli.

Abstract

Keywords

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