In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol

AE Bartram; MJ Winter; DB Huggett; P McCormack; LA Constantine; MJ Hetheridge; TH Hutchinson; LB Kinter; JF Ericson; JP Sumpter; SF Owen

doi:10.1002/tox.20684

In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol

AE Bartram, MJ Winter, DB Huggett, P McCormack, LA Constantine, MJ Hetheridge, TH Hutchinson, LB Kinter, JF Ericson, JP Sumpter, SF Owen

School of Geography, Earth and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, an in vivo time course exposure with 1 mg/L was conducted. Additionally, using measured in vivo plasma concentrations, an in vitro exposure at human therapeutic levels was undertaken. This allowed comparison of in vitro and in vivo rates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis. In vitro exposure of suspended liver and gill cells at concentrations similar to in vivo levels resulted in EROD activity in both tissues, but with significantly higher rates (up to six times in vivo levels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable both in vivo (albeit nonsignificantly in the liver) and in vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.

Original language	English
Pages (from-to)	573-582
Number of pages	0
Journal	Environmental Toxicology
Volume	27
Issue number	10
Early online date	7 Mar 2011
DOIs	https://doi.org/10.1002/tox.20684
Publication status	Published - Oct 2012

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Bartram, AE., Winter, MJ., Huggett, DB., McCormack, P., Constantine, LA., Hetheridge, MJ., Hutchinson, TH., Kinter, LB., Ericson, JF., Sumpter, JP., & Owen, SF. (2012). In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol. Environmental Toxicology, 27(10), 573-582. https://doi.org/10.1002/tox.20684

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title = "In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol",

abstract = "AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, an in vivo time course exposure with 1 mg/L was conducted. Additionally, using measured in vivo plasma concentrations, an in vitro exposure at human therapeutic levels was undertaken. This allowed comparison of in vitro and in vivo rates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis. In vitro exposure of suspended liver and gill cells at concentrations similar to in vivo levels resulted in EROD activity in both tissues, but with significantly higher rates (up to six times in vivo levels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable both in vivo (albeit nonsignificantly in the liver) and in vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. {\textcopyright} 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.",

author = "AE Bartram and MJ Winter and DB Huggett and P McCormack and LA Constantine and MJ Hetheridge and TH Hutchinson and LB Kinter and JF Ericson and JP Sumpter and SF Owen",

year = "2012",

month = oct,

doi = "10.1002/tox.20684",

language = "English",

volume = "27",

pages = "573--582",

journal = "Environmental Toxicology",

issn = "1520-4081",

publisher = "John Wiley & Sons Inc.",

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Bartram, AE, Winter, MJ, Huggett, DB, McCormack, P, Constantine, LA, Hetheridge, MJ, Hutchinson, TH, Kinter, LB, Ericson, JF, Sumpter, JP & Owen, SF 2012, 'In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol', Environmental Toxicology, vol. 27, no. 10, pp. 573-582. https://doi.org/10.1002/tox.20684

TY - JOUR

T1 - In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol

AU - Bartram, AE

AU - Winter, MJ

AU - Huggett, DB

AU - McCormack, P

AU - Constantine, LA

AU - Hetheridge, MJ

AU - Hutchinson, TH

AU - Kinter, LB

AU - Ericson, JF

AU - Sumpter, JP

AU - Owen, SF

PY - 2012/10

Y1 - 2012/10

N2 - AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, an in vivo time course exposure with 1 mg/L was conducted. Additionally, using measured in vivo plasma concentrations, an in vitro exposure at human therapeutic levels was undertaken. This allowed comparison of in vitro and in vivo rates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis. In vitro exposure of suspended liver and gill cells at concentrations similar to in vivo levels resulted in EROD activity in both tissues, but with significantly higher rates (up to six times in vivo levels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable both in vivo (albeit nonsignificantly in the liver) and in vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.

AB - AbstractThe conservation of common physiological systems across vertebrate classes suggests the potential for certain pharmaceuticals, which have been detected in surface waters, to produce biological effects in nontarget vertebrates such as fish. However, previous studies assessing the effects of such compounds in fish have not taken into account the potential for metabolism and elimination. This study aimed to assess if propranolol, a β‐adrenergic receptor antagonist or β‐blocker, could modulate EROD activity (indicative of CYP1A activity) in rainbow trout (Oncorhynchus mykiss) gills and liver. For this, an in vivo time course exposure with 1 mg/L was conducted. Additionally, using measured in vivo plasma concentrations, an in vitro exposure at human therapeutic levels was undertaken. This allowed comparison of in vitro and in vivo rates of EROD activity, thus investigating the applicability of cell preparations as surrogates for whole animal enzyme activity analysis. In vitro exposure of suspended liver and gill cells at concentrations similar to in vivo levels resulted in EROD activity in both tissues, but with significantly higher rates (up to six times in vivo levels). These results show that propranolol exposure elevated EROD activity in the liver and gill of rainbow trout, and that this is demonstrable both in vivo (albeit nonsignificantly in the liver) and in vitro, thus supporting the use of the latter as a surrogate of the former. These data also provide an insight into the potential role of the gill as a site of metabolism of pharmaceuticals in trout, suggesting that propranolol (and feasibly other pharmaceuticals) may undergo “first pass” metabolism in this organ. © 2011 Wiley Periodicals, Inc. Environ Toxicol 2012.

U2 - 10.1002/tox.20684

DO - 10.1002/tox.20684

M3 - Article

SN - 1520-4081

VL - 27

SP - 573

EP - 582

JO - Environmental Toxicology

JF - Environmental Toxicology

IS - 10

ER -

In vivo and in vitro liver and gill EROD activity in rainbow trout (Oncorhynchus mykiss) exposed to the beta‐blocker propranolol

Abstract

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