Graft Materials and Bone Marrow Stromal Cells in Bone Tissue Engineering

F Foschi; E Conserva; P Pera; B Canciani; R Cancedda; M Mastrogiacomo

doi:10.1177/0885328210393046

Graft Materials and Bone Marrow Stromal Cells in Bone Tissue Engineering

F Foschi, E Conserva, P Pera, B Canciani, R Cancedda, M Mastrogiacomo

Peninsula Dental School

Research output: Contribution to journal › Article › peer-review

Abstract

Bone augmentation procedures rely on osteogenic/osteoconductive properties of bone graft material (BGM). A further improvement is represented by use of autologous bone marrow stromal cells (BMSC), expanded in vitro and seeded on BGM before implantation in the bone defect. The effect of different BGMs on BMSC osteogenic differentiation was evaluated. BMSC were cultured in vitro in the presence of different BGM (natural, synthetic, and mixed origins). Cellular morphology was analyzed with scanning electron microscopy. The capability of BMSC to differentiate was determined in vitro by alkaline phosphatase gene expression and enzyme activity at different time points (7, 14, and 28 days) and in vivo by ectopic bone formation of implanted tissue constructs in an immunodeficient murine model. BGM supports the cell adhesion and osteogenic differentiation of BMSC developing a useful tool in the bone tissue engineering.

Original language	English
Pages (from-to)	1035-1049
Number of pages	0
Journal	Journal of Biomaterials Applications
Volume	26
Issue number	8
Early online date	1 Mar 2011
DOIs	https://doi.org/10.1177/0885328210393046
Publication status	Published - May 2012

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title = "Graft Materials and Bone Marrow Stromal Cells in Bone Tissue Engineering",

abstract = "Bone augmentation procedures rely on osteogenic/osteoconductive properties of bone graft material (BGM). A further improvement is represented by use of autologous bone marrow stromal cells (BMSC), expanded in vitro and seeded on BGM before implantation in the bone defect. The effect of different BGMs on BMSC osteogenic differentiation was evaluated. BMSC were cultured in vitro in the presence of different BGM (natural, synthetic, and mixed origins). Cellular morphology was analyzed with scanning electron microscopy. The capability of BMSC to differentiate was determined in vitro by alkaline phosphatase gene expression and enzyme activity at different time points (7, 14, and 28 days) and in vivo by ectopic bone formation of implanted tissue constructs in an immunodeficient murine model. BGM supports the cell adhesion and osteogenic differentiation of BMSC developing a useful tool in the bone tissue engineering.",

author = "F Foschi and E Conserva and P Pera and B Canciani and R Cancedda and M Mastrogiacomo",

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AU - Foschi, F

AU - Conserva, E

AU - Pera, P

AU - Canciani, B

AU - Cancedda, R

AU - Mastrogiacomo, M

PY - 2012/5

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AB - Bone augmentation procedures rely on osteogenic/osteoconductive properties of bone graft material (BGM). A further improvement is represented by use of autologous bone marrow stromal cells (BMSC), expanded in vitro and seeded on BGM before implantation in the bone defect. The effect of different BGMs on BMSC osteogenic differentiation was evaluated. BMSC were cultured in vitro in the presence of different BGM (natural, synthetic, and mixed origins). Cellular morphology was analyzed with scanning electron microscopy. The capability of BMSC to differentiate was determined in vitro by alkaline phosphatase gene expression and enzyme activity at different time points (7, 14, and 28 days) and in vivo by ectopic bone formation of implanted tissue constructs in an immunodeficient murine model. BGM supports the cell adhesion and osteogenic differentiation of BMSC developing a useful tool in the bone tissue engineering.

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JO - Journal of Biomaterials Applications

JF - Journal of Biomaterials Applications

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Graft Materials and Bone Marrow Stromal Cells in Bone Tissue Engineering

Abstract

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