G‐protein coupled receptor‐mediated nutrient sensing and developmental control in <scp>A</scp>spergillus nidulans

Neil Andrew Brown; Reis TF dos; Laure Nicolas Annick Ries; Camila Caldana; Jae Hyung Mah; Jae Hyuk Yu; Jeffrey M. Macdonald; Gustavo Henrique Goldman

doi:10.1111/mmi.13135

G‐protein coupled receptor‐mediated nutrient sensing and developmental control in <scp>A</scp>spergillus nidulans

Neil Andrew Brown^*, Reis TF dos, Laure Nicolas Annick Ries, Camila Caldana, Jae Hyung Mah, Jae Hyuk Yu, Jeffrey M. Macdonald, Gustavo Henrique Goldman

^*Corresponding author for this work

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

SummaryNutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms for sensing and taking up various nutrients. Despite its importance, only a limited number of nutrient receptors and their corresponding ligands have been identified in fungi. G‐protein coupled receptors (GPCRs) are the largest family of transmembrane receptors. The Aspergillus nidulans genome encodes 16 putative GPCRs, but only a few have been functionally characterised. Our previous study showed the increased expression of an uncharacterised putative GPCR, gprH, during carbon starvation. GprH appears conserved throughout numerous filamentous fungi. Here, we reveal that GprH is a putative receptor involved in glucose and tryptophan sensing. The absence of GprH results in a reduction in cAMP levels and PKA activity upon adding glucose or tryptophan to starved cells. GprH is pre‐formed in conidia and is increasingly active during carbon starvation, where it plays a role in glucose uptake and the recovery of hyphal growth. GprH also represses sexual development under conditions favouring sexual fruiting and during carbon starvation in submerged cultures. In summary, the GprH nutrient‐sensing system functions upstream of the cAMP‐PKA pathway, influences primary metabolism and hyphal growth, while represses sexual development in A. nidulans.

Original language	English
Pages (from-to)	420-439
Number of pages	0
Journal	Molecular Microbiology
Volume	98
Issue number	3
Early online date	15 Aug 2015
DOIs	https://doi.org/10.1111/mmi.13135
Publication status	Published - Oct 2015

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@article{f84fff969d6649758b71863a84957dda,

title = "G‐protein coupled receptor‐mediated nutrient sensing and developmental control in Aspergillus nidulans",

abstract = "SummaryNutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms for sensing and taking up various nutrients. Despite its importance, only a limited number of nutrient receptors and their corresponding ligands have been identified in fungi. G‐protein coupled receptors (GPCRs) are the largest family of transmembrane receptors. The Aspergillus nidulans genome encodes 16 putative GPCRs, but only a few have been functionally characterised. Our previous study showed the increased expression of an uncharacterised putative GPCR, gprH, during carbon starvation. GprH appears conserved throughout numerous filamentous fungi. Here, we reveal that GprH is a putative receptor involved in glucose and tryptophan sensing. The absence of GprH results in a reduction in cAMP levels and PKA activity upon adding glucose or tryptophan to starved cells. GprH is pre‐formed in conidia and is increasingly active during carbon starvation, where it plays a role in glucose uptake and the recovery of hyphal growth. GprH also represses sexual development under conditions favouring sexual fruiting and during carbon starvation in submerged cultures. In summary, the GprH nutrient‐sensing system functions upstream of the cAMP‐PKA pathway, influences primary metabolism and hyphal growth, while represses sexual development in A. nidulans.",

author = "Brown, {Neil Andrew} and dos, {Reis TF} and Ries, {Laure Nicolas Annick} and Camila Caldana and Mah, {Jae Hyung} and Yu, {Jae Hyuk} and Macdonald, {Jeffrey M.} and Goldman, {Gustavo Henrique}",

year = "2015",

month = oct,

doi = "10.1111/mmi.13135",

language = "English",

volume = "98",

pages = "420--439",

journal = "Molecular Microbiology",

issn = "0950-382X",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "3",

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TY - JOUR

T1 - G‐protein coupled receptor‐mediated nutrient sensing and developmental control in Aspergillus nidulans

AU - Brown, Neil Andrew

AU - dos, Reis TF

AU - Ries, Laure Nicolas Annick

AU - Caldana, Camila

AU - Mah, Jae Hyung

AU - Yu, Jae Hyuk

AU - Macdonald, Jeffrey M.

AU - Goldman, Gustavo Henrique

PY - 2015/10

Y1 - 2015/10

N2 - SummaryNutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms for sensing and taking up various nutrients. Despite its importance, only a limited number of nutrient receptors and their corresponding ligands have been identified in fungi. G‐protein coupled receptors (GPCRs) are the largest family of transmembrane receptors. The Aspergillus nidulans genome encodes 16 putative GPCRs, but only a few have been functionally characterised. Our previous study showed the increased expression of an uncharacterised putative GPCR, gprH, during carbon starvation. GprH appears conserved throughout numerous filamentous fungi. Here, we reveal that GprH is a putative receptor involved in glucose and tryptophan sensing. The absence of GprH results in a reduction in cAMP levels and PKA activity upon adding glucose or tryptophan to starved cells. GprH is pre‐formed in conidia and is increasingly active during carbon starvation, where it plays a role in glucose uptake and the recovery of hyphal growth. GprH also represses sexual development under conditions favouring sexual fruiting and during carbon starvation in submerged cultures. In summary, the GprH nutrient‐sensing system functions upstream of the cAMP‐PKA pathway, influences primary metabolism and hyphal growth, while represses sexual development in A. nidulans.

AB - SummaryNutrient sensing and utilisation are fundamental for all life forms. As heterotrophs, fungi have evolved a diverse range of mechanisms for sensing and taking up various nutrients. Despite its importance, only a limited number of nutrient receptors and their corresponding ligands have been identified in fungi. G‐protein coupled receptors (GPCRs) are the largest family of transmembrane receptors. The Aspergillus nidulans genome encodes 16 putative GPCRs, but only a few have been functionally characterised. Our previous study showed the increased expression of an uncharacterised putative GPCR, gprH, during carbon starvation. GprH appears conserved throughout numerous filamentous fungi. Here, we reveal that GprH is a putative receptor involved in glucose and tryptophan sensing. The absence of GprH results in a reduction in cAMP levels and PKA activity upon adding glucose or tryptophan to starved cells. GprH is pre‐formed in conidia and is increasingly active during carbon starvation, where it plays a role in glucose uptake and the recovery of hyphal growth. GprH also represses sexual development under conditions favouring sexual fruiting and during carbon starvation in submerged cultures. In summary, the GprH nutrient‐sensing system functions upstream of the cAMP‐PKA pathway, influences primary metabolism and hyphal growth, while represses sexual development in A. nidulans.

U2 - 10.1111/mmi.13135

DO - 10.1111/mmi.13135

M3 - Article

SN - 0950-382X

VL - 98

SP - 420

EP - 439

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 3

ER -

G‐protein coupled receptor‐mediated nutrient sensing and developmental control in <scp><i>A</i></scp><i>spergillus nidulans</i>

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