TY - JOUR
T1 - Functionalization of Graphene Oxide for Label-Free Electrochemical Detection of Hepatic Cancer Cells
AU - Damiati, Samar
AU - Awan, Shakil A.
AU - Peacock, Martin
AU - Schuster, Bernhard
PY - 2023/5/12
Y1 - 2023/5/12
N2 - : In this study, a graphene oxide-modified screen-printed carbon electrode was functionalized
with the 1-pyrenebutyric acid-N-hydroxy-succinimide ester and conjugated with antibodies for the
label-free detection of human hepatoma HepG2 cells. Using a user-friendly reservoir chamber, the
functionalized film was exposed continuously to the cancer cells. The use of a continuous flow was
intended to enhance the capture of the target cells by the sensing platform. The response of the
biosensor was evaluated using cyclic voltammetry. The preliminary data showed good sensitivity for
the detection of hepatic cancer cells. The developed biosensor could detect the HepG2 cells from a
1 × 103
to 3 × 105
cells/mL range. Thus, it is a simple tool for the electrochemical detection of cancer
cells and offers a low-cost and disposable sensing platform.
AB - : In this study, a graphene oxide-modified screen-printed carbon electrode was functionalized
with the 1-pyrenebutyric acid-N-hydroxy-succinimide ester and conjugated with antibodies for the
label-free detection of human hepatoma HepG2 cells. Using a user-friendly reservoir chamber, the
functionalized film was exposed continuously to the cancer cells. The use of a continuous flow was
intended to enhance the capture of the target cells by the sensing platform. The response of the
biosensor was evaluated using cyclic voltammetry. The preliminary data showed good sensitivity for
the detection of hepatic cancer cells. The developed biosensor could detect the HepG2 cells from a
1 × 103
to 3 × 105
cells/mL range. Thus, it is a simple tool for the electrochemical detection of cancer
cells and offers a low-cost and disposable sensing platform.
UR - https://pearl.plymouth.ac.uk/secam-research/1136/
U2 - 10.3390/IECB2023-14599
DO - 10.3390/IECB2023-14599
M3 - Conference proceedings published in a journal
SN - 2673-4591
VL - 35
JO - Engineering Proceedings
JF - Engineering Proceedings
IS - 1
T2 - 3rd International Electronic Conference on Biosensors IECB
Y2 - 8 May 2023 through 21 May 2023
ER -
