Functional imaging in living plants—cell biology meets physiology

The last quarter of a century has brought major developments in the acquisition of images from plants through improvements in microscopy equipment, software and technique. Likewise, step changes in image analysis tools and the utilization and iterative redesign of fluorescent protein based markers and probes has revolutionized the ability of researchers to ask fundamental questions in cell biology and physiology. This research topic gives a snapshot of the current shape of the field in the plant sciences.