Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans

Rafael Wesley Bastos; Clara Valero; Lilian Pereira Silva; Taylor Schoen; Milton Drott; Verônica Brauer; Rafael Silva-Rocha; Abigail Lind; Jacob L. Steenwyk; Antonis Rokas; Fernando Rodrigues; Agustin Resendiz-Sharpe; Katrien Lagrou; Marina Marcet-Houben; Toni Gabaldón; Erin McDonnell; Ian Reid; Adrian Tsang; Berl R. Oakley; Flávio Vieira Loures; Fausto Almeida; Anna Huttenlocher; Nancy P. Keller; Laure Nicolas Annick Ries; Gustavo H. Goldman

doi:10.1128/msphere.00153-20

Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans

Rafael Wesley Bastos, Clara Valero, Lilian Pereira Silva, Taylor Schoen, Milton Drott, Verônica Brauer, Rafael Silva-Rocha, Abigail Lind, Jacob L. Steenwyk, Antonis Rokas, Fernando Rodrigues, Agustin Resendiz-Sharpe, Katrien Lagrou, Marina Marcet-Houben, Toni Gabaldón, Erin McDonnell, Ian Reid, Adrian Tsang, Berl R. Oakley, Flávio Vieira LouresFausto Almeida, Anna Huttenlocher, Nancy P. Keller, Laure Nicolas Annick Ries^*, Gustavo H. Goldman^*

^*Corresponding author for this work

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Immunocompromised patients are susceptible to infections with opportunistic filamentous fungi from the genus Aspergillus . Although A. fumigatus is the main etiological agent of Aspergillus species-related infections, other species, such as A. nidulans , are prevalent in a condition-specific manner. A. nidulans is a predominant infective agent in patients suffering from chronic granulomatous disease (CGD). A. nidulans isolates have mainly been studied in the context of CGD although infection with A. nidulans also occurs in non-CGD patients. This study carried out a detailed biological characterization of two non-CGD A. nidulans clinical isolates and compared the results to those with a reference strain. Phenotypic, metabolomic, and genomic analyses highlight fundamental differences in carbon source utilization, stress responses, and maintenance of cell wall integrity among the strains. One clinical strain had increased virulence in models with impaired neutrophil function. Just as in A. fumigatus , strain heterogeneity exists in A. nidulans clinical strains that can define virulence traits.

Original language	English
Number of pages	0
Journal	mSphere
Volume	5
Issue number	2
DOIs	https://doi.org/10.1128/msphere.00153-20
Publication status	Published - 29 Apr 2020

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Bastos, R. W., Valero, C., Silva, L. P., Schoen, T., Drott, M., Brauer, V., Silva-Rocha, R., Lind, A., Steenwyk, J. L., Rokas, A., Rodrigues, F., Resendiz-Sharpe, A., Lagrou, K., Marcet-Houben, M., Gabaldón, T., McDonnell, E., Reid, I., Tsang, A., Oakley, B. R., ... Goldman, G. H. (2020). Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans. mSphere, 5(2). https://doi.org/10.1128/msphere.00153-20

@article{3702c28b184b40d5bdb578449709edb7,

title = "Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans",

abstract = " Immunocompromised patients are susceptible to infections with opportunistic filamentous fungi from the genus Aspergillus . Although A. fumigatus is the main etiological agent of Aspergillus species-related infections, other species, such as A. nidulans , are prevalent in a condition-specific manner. A. nidulans is a predominant infective agent in patients suffering from chronic granulomatous disease (CGD). A. nidulans isolates have mainly been studied in the context of CGD although infection with A. nidulans also occurs in non-CGD patients. This study carried out a detailed biological characterization of two non-CGD A. nidulans clinical isolates and compared the results to those with a reference strain. Phenotypic, metabolomic, and genomic analyses highlight fundamental differences in carbon source utilization, stress responses, and maintenance of cell wall integrity among the strains. One clinical strain had increased virulence in models with impaired neutrophil function. Just as in A. fumigatus , strain heterogeneity exists in A. nidulans clinical strains that can define virulence traits. ",

author = "Bastos, {Rafael Wesley} and Clara Valero and Silva, {Lilian Pereira} and Taylor Schoen and Milton Drott and Ver{\^o}nica Brauer and Rafael Silva-Rocha and Abigail Lind and Steenwyk, {Jacob L.} and Antonis Rokas and Fernando Rodrigues and Agustin Resendiz-Sharpe and Katrien Lagrou and Marina Marcet-Houben and Toni Gabald{\'o}n and Erin McDonnell and Ian Reid and Adrian Tsang and Oakley, {Berl R.} and Loures, {Fl{\'a}vio Vieira} and Fausto Almeida and Anna Huttenlocher and Keller, {Nancy P.} and Ries, {Laure Nicolas Annick} and Goldman, {Gustavo H.}",

year = "2020",

month = apr,

day = "29",

doi = "10.1128/msphere.00153-20",

language = "English",

volume = "5",

journal = "mSphere",

issn = "2379-5042",

publisher = "American Society for Microbiology",

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}

Bastos, RW, Valero, C, Silva, LP, Schoen, T, Drott, M, Brauer, V, Silva-Rocha, R, Lind, A, Steenwyk, JL, Rokas, A, Rodrigues, F, Resendiz-Sharpe, A, Lagrou, K, Marcet-Houben, M, Gabaldón, T, McDonnell, E, Reid, I, Tsang, A, Oakley, BR, Loures, FV, Almeida, F, Huttenlocher, A, Keller, NP, Ries, LNA & Goldman, GH 2020, 'Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans', mSphere, vol. 5, no. 2. https://doi.org/10.1128/msphere.00153-20

TY - JOUR

T1 - Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans

AU - Bastos, Rafael Wesley

AU - Valero, Clara

AU - Silva, Lilian Pereira

AU - Schoen, Taylor

AU - Drott, Milton

AU - Brauer, Verônica

AU - Silva-Rocha, Rafael

AU - Lind, Abigail

AU - Steenwyk, Jacob L.

AU - Rokas, Antonis

AU - Rodrigues, Fernando

AU - Resendiz-Sharpe, Agustin

AU - Lagrou, Katrien

AU - Marcet-Houben, Marina

AU - Gabaldón, Toni

AU - McDonnell, Erin

AU - Reid, Ian

AU - Tsang, Adrian

AU - Oakley, Berl R.

AU - Loures, Flávio Vieira

AU - Almeida, Fausto

AU - Huttenlocher, Anna

AU - Keller, Nancy P.

AU - Ries, Laure Nicolas Annick

AU - Goldman, Gustavo H.

PY - 2020/4/29

Y1 - 2020/4/29

N2 - Immunocompromised patients are susceptible to infections with opportunistic filamentous fungi from the genus Aspergillus . Although A. fumigatus is the main etiological agent of Aspergillus species-related infections, other species, such as A. nidulans , are prevalent in a condition-specific manner. A. nidulans is a predominant infective agent in patients suffering from chronic granulomatous disease (CGD). A. nidulans isolates have mainly been studied in the context of CGD although infection with A. nidulans also occurs in non-CGD patients. This study carried out a detailed biological characterization of two non-CGD A. nidulans clinical isolates and compared the results to those with a reference strain. Phenotypic, metabolomic, and genomic analyses highlight fundamental differences in carbon source utilization, stress responses, and maintenance of cell wall integrity among the strains. One clinical strain had increased virulence in models with impaired neutrophil function. Just as in A. fumigatus , strain heterogeneity exists in A. nidulans clinical strains that can define virulence traits.

AB - Immunocompromised patients are susceptible to infections with opportunistic filamentous fungi from the genus Aspergillus . Although A. fumigatus is the main etiological agent of Aspergillus species-related infections, other species, such as A. nidulans , are prevalent in a condition-specific manner. A. nidulans is a predominant infective agent in patients suffering from chronic granulomatous disease (CGD). A. nidulans isolates have mainly been studied in the context of CGD although infection with A. nidulans also occurs in non-CGD patients. This study carried out a detailed biological characterization of two non-CGD A. nidulans clinical isolates and compared the results to those with a reference strain. Phenotypic, metabolomic, and genomic analyses highlight fundamental differences in carbon source utilization, stress responses, and maintenance of cell wall integrity among the strains. One clinical strain had increased virulence in models with impaired neutrophil function. Just as in A. fumigatus , strain heterogeneity exists in A. nidulans clinical strains that can define virulence traits.

U2 - 10.1128/msphere.00153-20

DO - 10.1128/msphere.00153-20

M3 - Article

SN - 2379-5042

VL - 5

JO - mSphere

JF - mSphere

IS - 2

ER -

Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans

Abstract

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