Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Roy M. Pemberton; Timothy Cox; Rachel Tuffin; Guido A. Drago; John Griffiths; Robin Pittson; Graham Johnson; Jinsheng Xu; Ian C. Sage; Rhodri Davies; Simon K. Jackson; Gerry Kenna; Richard Luxton; John P. Hart

doi:10.3390/s141120519

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Roy M. Pemberton^*, Timothy Cox, Rachel Tuffin, Guido A. Drago, John Griffiths, Robin Pittson, Graham Johnson, Jinsheng Xu, Ian C. Sage, Rhodri Davies, Simon K. Jackson, Gerry Kenna, Richard Luxton, John P. Hart

^*Corresponding author for this work

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Abstract

This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O₂) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

Original language	English
Pages (from-to)	20519-20532
Number of pages	14
Journal	Sensors (Switzerland)
Volume	14
Issue number	11
DOIs	https://doi.org/10.3390/s141120519
Publication status	Published - 30 Oct 2014

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

Keywords

96-well plate
Amperometry
Cell monitoring
MEMS
Metabolism
Microbiosensor
Screen-printing
Toxicity testing

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10.3390/s141120519

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

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Pemberton, R. M., Cox, T., Tuffin, R., Drago, G. A., Griffiths, J., Pittson, R., Johnson, G., Xu, J., Sage, I. C., Davies, R., Jackson, S. K., Kenna, G., Luxton, R., & Hart, J. P. (2014). Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers. Sensors (Switzerland), 14(11), 20519-20532. https://doi.org/10.3390/s141120519

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title = "Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers",

abstract = "This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.",

keywords = "96-well plate, Amperometry, Cell monitoring, MEMS, Metabolism, Microbiosensor, Screen-printing, Toxicity testing",

author = "Pemberton, {Roy M.} and Timothy Cox and Rachel Tuffin and Drago, {Guido A.} and John Griffiths and Robin Pittson and Graham Johnson and Jinsheng Xu and Sage, {Ian C.} and Rhodri Davies and Jackson, {Simon K.} and Gerry Kenna and Richard Luxton and Hart, {John P.}",

note = "Publisher Copyright: {\textcopyright} 2014 by the authors; licensee MDPI, Basel, Switzerland.",

year = "2014",

month = oct,

day = "30",

doi = "10.3390/s141120519",

language = "English",

volume = "14",

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Pemberton, RM, Cox, T, Tuffin, R, Drago, GA, Griffiths, J, Pittson, R, Johnson, G, Xu, J, Sage, IC, Davies, R, Jackson, SK, Kenna, G, Luxton, R & Hart, JP 2014, 'Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers', Sensors (Switzerland), vol. 14, no. 11, pp. 20519-20532. https://doi.org/10.3390/s141120519

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AU - Pemberton, Roy M.

AU - Cox, Timothy

AU - Tuffin, Rachel

AU - Drago, Guido A.

AU - Griffiths, John

AU - Pittson, Robin

AU - Johnson, Graham

AU - Xu, Jinsheng

AU - Sage, Ian C.

AU - Davies, Rhodri

AU - Jackson, Simon K.

AU - Kenna, Gerry

AU - Luxton, Richard

AU - Hart, John P.

PY - 2014/10/30

Y1 - 2014/10/30

N2 - This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

AB - This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies.

KW - 96-well plate

KW - Amperometry

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KW - MEMS

KW - Metabolism

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ER -

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

Abstract

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Keywords

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