TY - JOUR
T1 - Exercise dose affects the circulating microRNA profile in response to acute endurance exercise in male amateur runners
AU - Fernández-Sanjurjo, Manuel
AU - Úbeda, Natalia
AU - Fernández-García, Benjamín
AU - del Valle, Miguel
AU - Ramírez de Molina, Ana
AU - Crespo, María Carmen
AU - Martín-Hernández, Roberto
AU - Casas-Agustench, Patricia
AU - Martínez-Camblor, Pablo
AU - de Gonzalo-Calvo, David
AU - Díez-Robles, Sergio
AU - García-González, Ángela
AU - Montero, Ana
AU - González-González, Felipe
AU - Rabadán, Manuel
AU - Díaz-Martínez, Ángel Enrique
AU - Whitham, Martin
AU - Iglesias-Gutiérrez, Eduardo
AU - Dávalos, Alberto
N1 - Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2020/10/1
Y1 - 2020/10/1
N2 - The systemic response to exercise is dose-dependent and involves a complex gene expression regulation and cross-talk between tissues. This context ARISES the need for analyzing the influence of exercise dose on the profile of circulating microRNAs (c-miRNAs), as emerging posttranscriptional regulators and intercellular communicators. Thus, we hypothesized that different exercise doses will determine specific c-miRNA signatures that will highlight its potential as exercise dose biomarker. Nine active middle-aged males completed a 10-km race (10K), a half-marathon (HM), and a marathon (M). Blood samples were collected immediately before and after races. Plasma RNA was extracted, and a global screening of 752 microRNAs was analyzed using RT-qPCR. Three different c-miRNA profiles were defined according to the three doses. In 10K, 14 c-miRNAs were found to be differentially expressed between pre- and post-exercise, 13 upregulated and 1 downregulated. Regarding HM, 13 c-miRNAs were found to be differentially modulated, in all the cases upregulated. A total of 28 c-miRNAs were found to be differentially expressed in M, 21 overexpressed and 7 repressed after this race. We had also found 3 common c-miRNAs between 10K and M and 2 common c-miRNAs between 10K and HM. In silico analysis supported a close association between exercise dose c-miRNA profiles and cellular pathways linked to energy metabolism and cell cycle. In conclusion, we have observed that different exercise doses induced specific c-miRNA profiles. So, our results point to c-miRNAs as emerging exercise dose biomarkers and as one of regulatory mechanisms modulating the response to endurance exercise.
AB - The systemic response to exercise is dose-dependent and involves a complex gene expression regulation and cross-talk between tissues. This context ARISES the need for analyzing the influence of exercise dose on the profile of circulating microRNAs (c-miRNAs), as emerging posttranscriptional regulators and intercellular communicators. Thus, we hypothesized that different exercise doses will determine specific c-miRNA signatures that will highlight its potential as exercise dose biomarker. Nine active middle-aged males completed a 10-km race (10K), a half-marathon (HM), and a marathon (M). Blood samples were collected immediately before and after races. Plasma RNA was extracted, and a global screening of 752 microRNAs was analyzed using RT-qPCR. Three different c-miRNA profiles were defined according to the three doses. In 10K, 14 c-miRNAs were found to be differentially expressed between pre- and post-exercise, 13 upregulated and 1 downregulated. Regarding HM, 13 c-miRNAs were found to be differentially modulated, in all the cases upregulated. A total of 28 c-miRNAs were found to be differentially expressed in M, 21 overexpressed and 7 repressed after this race. We had also found 3 common c-miRNAs between 10K and M and 2 common c-miRNAs between 10K and HM. In silico analysis supported a close association between exercise dose c-miRNA profiles and cellular pathways linked to energy metabolism and cell cycle. In conclusion, we have observed that different exercise doses induced specific c-miRNA profiles. So, our results point to c-miRNAs as emerging exercise dose biomarkers and as one of regulatory mechanisms modulating the response to endurance exercise.
KW - circulating microRNAs
KW - endurance exercise
KW - exercise biomarkers
KW - exercise dose
UR - http://www.scopus.com/inward/record.url?scp=85088295015&partnerID=8YFLogxK
U2 - 10.1111/sms.13759
DO - 10.1111/sms.13759
M3 - Article
C2 - 32609897
AN - SCOPUS:85088295015
SN - 0905-7188
VL - 30
SP - 1896
EP - 1907
JO - Scandinavian Journal of Medicine and Science in Sports
JF - Scandinavian Journal of Medicine and Science in Sports
IS - 10
ER -