TY - JOUR
T1 - Evaluation of the genus Thiothrix Winogradsky 1888 (Approved Lists 1980) emend. Aruga et al. 2002: reclassification of Thiothrix disciformis to Thiolinea disciformis gen. nov., comb. nov., and of Thiothrix flexilis to Thiofilum flexile gen. nov., comb nov., with emended description of Thiothrix.
AU - Boden, Rich
AU - Scott, Kathleen M.
PY - 2018/5/31
Y1 - 2018/5/31
N2 - Thiothrix is the type genus of the Thiotrichaceae in the Thiotrichales of the
21 Gammaproteobacteria, comprising nine species of sulfur-oxidising filamentous Bacteria,
22 which are variously autotrophic, heterotrophic or have mixed metabolic modes. Within the
23 genus, 4 species show 16S rRNA gene identities lower the Yarza threshold for the rank of
24 genus (94.5 %) – Thiothrix disciformis, Thiothrix flexilis, Thiothrix defluvii and Thiothrix
25 eikelboomii – as they show no affiliation to extant genera, a polyphasic study was undertaken
26 including biochemical, physiological and genomic properties and phylogeny based on the
27 16S rRNA gene (rrs), recombination protein A (RecA), polynucleotide nucleotidyltransferase
28 (Pnp), translation initiation factor IF-2 (InfB), glyceraldehyde-3-phosphate dehydrogenase
29 (GapA), glutaminyl-tRNA synthetase (GlnS), elongation factor EF-G (FusA) and
30 concatamers of 53 ribosomal proteins encoded by rps, rpl and rpm operons, all of which
31 support the reclassification of these species. We thus propose Thiolinea gen. nov. and
32 Thiofilum gen. nov. for which the type species are Thiolinea disciformis gen. nov., comb.
33 nov. and Thiofilum flexile gen. nov., comb. nov. We also propose that these genera are each
34 circumscribed into novel families Thiolinaceae fam. nov. and Thiofilaceae fam. nov., and
35 that Leucothrix and Cocleimonas are circumscribed into Leucotrichaceae fam. nov. and
36 provide emended descriptions of Thiothrix and Thiotrichaceae.
AB - Thiothrix is the type genus of the Thiotrichaceae in the Thiotrichales of the
21 Gammaproteobacteria, comprising nine species of sulfur-oxidising filamentous Bacteria,
22 which are variously autotrophic, heterotrophic or have mixed metabolic modes. Within the
23 genus, 4 species show 16S rRNA gene identities lower the Yarza threshold for the rank of
24 genus (94.5 %) – Thiothrix disciformis, Thiothrix flexilis, Thiothrix defluvii and Thiothrix
25 eikelboomii – as they show no affiliation to extant genera, a polyphasic study was undertaken
26 including biochemical, physiological and genomic properties and phylogeny based on the
27 16S rRNA gene (rrs), recombination protein A (RecA), polynucleotide nucleotidyltransferase
28 (Pnp), translation initiation factor IF-2 (InfB), glyceraldehyde-3-phosphate dehydrogenase
29 (GapA), glutaminyl-tRNA synthetase (GlnS), elongation factor EF-G (FusA) and
30 concatamers of 53 ribosomal proteins encoded by rps, rpl and rpm operons, all of which
31 support the reclassification of these species. We thus propose Thiolinea gen. nov. and
32 Thiofilum gen. nov. for which the type species are Thiolinea disciformis gen. nov., comb.
33 nov. and Thiofilum flexile gen. nov., comb. nov. We also propose that these genera are each
34 circumscribed into novel families Thiolinaceae fam. nov. and Thiofilaceae fam. nov., and
35 that Leucothrix and Cocleimonas are circumscribed into Leucotrichaceae fam. nov. and
36 provide emended descriptions of Thiothrix and Thiotrichaceae.
KW - Thiothrix
KW - Thiotrichales
KW - Gammaproteobacteria
KW - Thiolinea
KW - Thiofilum
U2 - 10.1099/ijsem.0.002816
DO - 10.1099/ijsem.0.002816
M3 - Article
SN - 1466-5026
VL - 0
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 0
ER -