Evaluation of the BAX® system for detection of Listeria monocytogenes in foods: Collaborative study

Karen Silbernagel; Robert Jechorek; W. Mark Barbour; Peter Mrozinski; W. Alejo; V. Aleo; B. Andaloro; F. Beacorn; J. Benzinger; S. Bogar; C. Brayman; J. Broom; M. Carson; C. Carver; C. Cheng; B. Centrella; J. Clayborn; C. Collins; C. Deibel; M. Divine; S. Eliasberg; D. Farmer; S. Frye; T. Gatesy; E. Goodstein; C. Halker; G. Hall; P. Hanson; G. Hartman; K. Heddaeus; J. Hembree; J. Hutchins; P. Istafanos; R. Jechorek; J. Jenkins; K. Kerdahi; S. Kremer; A. Lal; S. Leighton; D. Lester; J. Lewis; J. Lin; J. Martin; M. Maselli; P. McCarthy; B. McGovern; M. Mills; F. Mohnke; B. Moon; D. Moss; M. Plaza; S. Robeson; H. Romero; D. Rubalcaba; A. Schultz; J. Seehusen; C. Shaw; K. Siem; E. Sloan; J. Stanerson; N. Stepanova; K. Van; K. Van Enkenvoort; M. Vialpando; W. Warren; K. Watts; K. Wilson; T. Woodruff

Evaluation of the BAX® system for detection of Listeria monocytogenes in foods: Collaborative study

Karen Silbernagel^*
, Robert Jechorek
, W. Mark Barbour
, Peter Mrozinski
, W. Alejo
, V. Aleo
, B. Andaloro
, F. Beacorn
, J. Benzinger
, S. Bogar
, C. Brayman
, J. Broom
, M. Carson
, C. Carver
, C. Cheng
, B. Centrella
, J. Clayborn
, C. Collins
, C. Deibel
, M. Divine

S. Eliasberg, D. Farmer, S. Frye, T. Gatesy, E. Goodstein, C. Halker, G. Hall, P. Hanson, G. Hartman, K. Heddaeus, J. Hembree, J. Hutchins, P. Istafanos, R. Jechorek, J. Jenkins, K. Kerdahi, S. Kremer, A. Lal, S. Leighton, D. Lester, J. Lewis, J. Lin, J. Martin, M. Maselli, P. McCarthy, B. McGovern, M. Mills, F. Mohnke, B. Moon, D. Moss, M. Plaza, S. Robeson, H. Romero, D. Rubalcaba, A. Schultz, J. Seehusen, C. Shaw, K. Siem, E. Sloan, J. Stanerson, N. Stepanova, K. Van, K. Van Enkenvoort, M. Vialpando, W. Warren, K. Watts, K. Wilson, T. Woodruff

^*Corresponding author for this work

School of Biological and Marine Sciences

rtech laboratories
DuPont

Research output: Contribution to journal › Article › peer-review

Abstract

A multilaboratory study was conducted to compare the automated BAX® system and the standard cultural methods for detection of Listeria monocytogenes in foods. Six food types (frankfurters, soft cheese, smoked salmon, raw, ground beef, fresh radishes, and frozen peas) were analyzed by each method. For each food type, 3 inoculation levels were tested: high (average of 2 CFU/g), low (average of 0.2 CFU/g) and uninoculated controls. A total of 25 laboratories representing government and industry participated. Of the 2335 samples analyzed, 1109 were positive by the BAX system and 1115 were positive by the standard method. A Chi square analysis of each of the 6 food types, at the 3 inoculation levels tested, was performed. For all foods, except radishes, the BAX system performed as well as or better than the standard reference methods based on the Chi square results.

Original language	English
Pages (from-to)	395-410
Number of pages	16
Journal	Journal of AOAC International
Volume	87
Issue number	2
Publication status	Published - Mar 2004

ASJC Scopus subject areas

Analytical Chemistry
Food Science
Environmental Chemistry
Agronomy and Crop Science
Pharmacology

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https://www.scopus.com/pages/publications/2542458494

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Silbernagel, K., Jechorek, R., Barbour, W. M., Mrozinski, P., Alejo, W., Aleo, V., Andaloro, B., Beacorn, F., Benzinger, J., Bogar, S., Brayman, C., Broom, J., Carson, M., Carver, C., Cheng, C., Centrella, B., Clayborn, J., Collins, C., Deibel, C., ... Woodruff, T. (2004). Evaluation of the BAX® system for detection of Listeria monocytogenes in foods: Collaborative study. Journal of AOAC International, 87(2), 395-410. https://www.scopus.com/pages/publications/2542458494

@article{0d962193c505458087bf25cf32edc048,

title = "Evaluation of the BAX{\textregistered} system for detection of Listeria monocytogenes in foods: Collaborative study",

abstract = "A multilaboratory study was conducted to compare the automated BAX{\textregistered} system and the standard cultural methods for detection of Listeria monocytogenes in foods. Six food types (frankfurters, soft cheese, smoked salmon, raw, ground beef, fresh radishes, and frozen peas) were analyzed by each method. For each food type, 3 inoculation levels were tested: high (average of 2 CFU/g), low (average of 0.2 CFU/g) and uninoculated controls. A total of 25 laboratories representing government and industry participated. Of the 2335 samples analyzed, 1109 were positive by the BAX system and 1115 were positive by the standard method. A Chi square analysis of each of the 6 food types, at the 3 inoculation levels tested, was performed. For all foods, except radishes, the BAX system performed as well as or better than the standard reference methods based on the Chi square results.",

author = "Karen Silbernagel and Robert Jechorek and Barbour, \{W. Mark\} and Peter Mrozinski and W. Alejo and V. Aleo and B. Andaloro and F. Beacorn and J. Benzinger and S. Bogar and C. Brayman and J. Broom and M. Carson and C. Carver and C. Cheng and B. Centrella and J. Clayborn and C. Collins and C. Deibel and M. Divine and S. Eliasberg and D. Farmer and S. Frye and T. Gatesy and E. Goodstein and C. Halker and G. Hall and P. Hanson and G. Hartman and K. Heddaeus and J. Hembree and J. Hutchins and P. Istafanos and R. Jechorek and J. Jenkins and K. Kerdahi and S. Kremer and A. Lal and S. Leighton and D. Lester and J. Lewis and J. Lin and J. Martin and M. Maselli and P. McCarthy and B. McGovern and M. Mills and F. Mohnke and B. Moon and D. Moss and M. Plaza and S. Robeson and H. Romero and D. Rubalcaba and A. Schultz and J. Seehusen and C. Shaw and K. Siem and E. Sloan and J. Stanerson and N. Stepanova and K. Van and \{Van Enkenvoort\}, K. and M. Vialpando and W. Warren and K. Watts and K. Wilson and T. Woodruff",

year = "2004",

month = mar,

language = "English",

volume = "87",

pages = "395--410",

journal = "Journal of AOAC International",

issn = "1060-3271",

publisher = "Oxford University Press",

number = "2",

}

Silbernagel, K, Jechorek, R, Barbour, WM, Mrozinski, P, Alejo, W, Aleo, V, Andaloro, B, Beacorn, F, Benzinger, J, Bogar, S, Brayman, C, Broom, J, Carson, M, Carver, C, Cheng, C, Centrella, B, Clayborn, J, Collins, C, Deibel, C, Divine, M, Eliasberg, S, Farmer, D, Frye, S, Gatesy, T, Goodstein, E, Halker, C, Hall, G, Hanson, P, Hartman, G, Heddaeus, K, Hembree, J, Hutchins, J, Istafanos, P, Jechorek, R, Jenkins, J, Kerdahi, K, Kremer, S, Lal, A, Leighton, S, Lester, D, Lewis, J, Lin, J, Martin, J, Maselli, M, McCarthy, P, McGovern, B, Mills, M, Mohnke, F, Moon, B, Moss, D, Plaza, M, Robeson, S, Romero, H, Rubalcaba, D, Schultz, A, Seehusen, J, Shaw, C, Siem, K, Sloan, E, Stanerson, J, Stepanova, N, Van, K, Van Enkenvoort, K, Vialpando, M, Warren, W, Watts, K, Wilson, K & Woodruff, T 2004, 'Evaluation of the BAX® system for detection of Listeria monocytogenes in foods: Collaborative study', Journal of AOAC International, vol. 87, no. 2, pp. 395-410. <https://www.scopus.com/pages/publications/2542458494>

TY - JOUR

T1 - Evaluation of the BAX® system for detection of Listeria monocytogenes in foods

T2 - Collaborative study

AU - Silbernagel, Karen

AU - Jechorek, Robert

AU - Barbour, W. Mark

AU - Mrozinski, Peter

AU - Alejo, W.

AU - Aleo, V.

AU - Andaloro, B.

AU - Beacorn, F.

AU - Benzinger, J.

AU - Bogar, S.

AU - Brayman, C.

AU - Broom, J.

AU - Carson, M.

AU - Carver, C.

AU - Cheng, C.

AU - Centrella, B.

AU - Clayborn, J.

AU - Collins, C.

AU - Deibel, C.

AU - Divine, M.

AU - Eliasberg, S.

AU - Farmer, D.

AU - Frye, S.

AU - Gatesy, T.

AU - Goodstein, E.

AU - Halker, C.

AU - Hall, G.

AU - Hanson, P.

AU - Hartman, G.

AU - Heddaeus, K.

AU - Hembree, J.

AU - Hutchins, J.

AU - Istafanos, P.

AU - Jechorek, R.

AU - Jenkins, J.

AU - Kerdahi, K.

AU - Kremer, S.

AU - Lal, A.

AU - Leighton, S.

AU - Lester, D.

AU - Lewis, J.

AU - Lin, J.

AU - Martin, J.

AU - Maselli, M.

AU - McCarthy, P.

AU - McGovern, B.

AU - Mills, M.

AU - Mohnke, F.

AU - Moon, B.

AU - Moss, D.

AU - Plaza, M.

AU - Robeson, S.

AU - Romero, H.

AU - Rubalcaba, D.

AU - Schultz, A.

AU - Seehusen, J.

AU - Shaw, C.

AU - Siem, K.

AU - Sloan, E.

AU - Stanerson, J.

AU - Stepanova, N.

AU - Van, K.

AU - Van Enkenvoort, K.

AU - Vialpando, M.

AU - Warren, W.

AU - Watts, K.

AU - Wilson, K.

AU - Woodruff, T.

PY - 2004/3

Y1 - 2004/3

N2 - A multilaboratory study was conducted to compare the automated BAX® system and the standard cultural methods for detection of Listeria monocytogenes in foods. Six food types (frankfurters, soft cheese, smoked salmon, raw, ground beef, fresh radishes, and frozen peas) were analyzed by each method. For each food type, 3 inoculation levels were tested: high (average of 2 CFU/g), low (average of 0.2 CFU/g) and uninoculated controls. A total of 25 laboratories representing government and industry participated. Of the 2335 samples analyzed, 1109 were positive by the BAX system and 1115 were positive by the standard method. A Chi square analysis of each of the 6 food types, at the 3 inoculation levels tested, was performed. For all foods, except radishes, the BAX system performed as well as or better than the standard reference methods based on the Chi square results.

AB - A multilaboratory study was conducted to compare the automated BAX® system and the standard cultural methods for detection of Listeria monocytogenes in foods. Six food types (frankfurters, soft cheese, smoked salmon, raw, ground beef, fresh radishes, and frozen peas) were analyzed by each method. For each food type, 3 inoculation levels were tested: high (average of 2 CFU/g), low (average of 0.2 CFU/g) and uninoculated controls. A total of 25 laboratories representing government and industry participated. Of the 2335 samples analyzed, 1109 were positive by the BAX system and 1115 were positive by the standard method. A Chi square analysis of each of the 6 food types, at the 3 inoculation levels tested, was performed. For all foods, except radishes, the BAX system performed as well as or better than the standard reference methods based on the Chi square results.

M3 - Article

C2 - 15164834

AN - SCOPUS:2542458494

SN - 1060-3271

VL - 87

SP - 395

EP - 410

JO - Journal of AOAC International

JF - Journal of AOAC International

IS - 2

ER -

Evaluation of the BAX® system for detection of Listeria monocytogenes in foods: Collaborative study

Abstract

ASJC Scopus subject areas

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