Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources.

Jian Liu; Louise J. Pankhurst; Lewis J. Deacon; Wondwossen Abate; Enda T. Hayes; Gill H. Drew; PJ Longhurst; Simon Pollard; J Longhurst; Sean F. Tyrrel; Simon K. Jackson

doi:10.1002/etc.434

Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources.

Jian Liu, Louise J. Pankhurst, Lewis J. Deacon, Wondwossen Abate, Enda T. Hayes, Gill H. Drew, PJ Longhurst, Simon Pollard, J Longhurst, Sean F. Tyrrel, Simon K. Jackson^*

^*Corresponding author for this work

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Because of the lack of effective methodology, the biological effects of environmental endotoxin have not been assessed. Here we have collected and measured airborne endotoxin at different locations around composting sites. Increased endotoxin concentrations were observed close to composting activities and also at nearby boundary areas. Analysis of proinflammatory effects of the environmental endotoxin on interleukin (IL)-8 and IL-6 release from human D562 pharyngeal epithelial and MM6 monocytic cell cultures showed an association between endotoxin level and cytokine induction. The cytokine-inducing effect of bioaerosol extracts was inhibited by polymyxin B, indicating that endotoxin was the cause of cytokine responses we found. The environmental endotoxin was also more active for stimulating cytokines in airway epithelial cells than commercially purified Escherichia coli endotoxin. Our results suggest that these in vitro inflammatory cell models may contribute to the assessment of health impacts of environmental endotoxin.

Original language	English
Pages (from-to)	602-606
Number of pages	0
Journal	Environ Toxicol Chem
Volume	30
Issue number	3
DOIs	https://doi.org/10.1002/etc.434
Publication status	Published - Mar 2011

Keywords

Air Pollutants
Biodegradation
Environmental
Cell Line
Endotoxins
Humans
Inflammation
Interleukin-6
Interleukin-8
Models
Biological
Respiratory Mucosa

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/etc.434

Cite this

@article{4153514f62694294bcce0387add3ca13,

title = "Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources.",

abstract = "Because of the lack of effective methodology, the biological effects of environmental endotoxin have not been assessed. Here we have collected and measured airborne endotoxin at different locations around composting sites. Increased endotoxin concentrations were observed close to composting activities and also at nearby boundary areas. Analysis of proinflammatory effects of the environmental endotoxin on interleukin (IL)-8 and IL-6 release from human D562 pharyngeal epithelial and MM6 monocytic cell cultures showed an association between endotoxin level and cytokine induction. The cytokine-inducing effect of bioaerosol extracts was inhibited by polymyxin B, indicating that endotoxin was the cause of cytokine responses we found. The environmental endotoxin was also more active for stimulating cytokines in airway epithelial cells than commercially purified Escherichia coli endotoxin. Our results suggest that these in vitro inflammatory cell models may contribute to the assessment of health impacts of environmental endotoxin.",

keywords = "Air Pollutants, Biodegradation, Environmental, Cell Line, Endotoxins, Humans, Inflammation, Interleukin-6, Interleukin-8, Models, Biological, Respiratory Mucosa",

author = "Jian Liu and Pankhurst, {Louise J.} and Deacon, {Lewis J.} and Wondwossen Abate and Hayes, {Enda T.} and Drew, {Gill H.} and PJ Longhurst and Simon Pollard and J Longhurst and Tyrrel, {Sean F.} and Jackson, {Simon K.}",

year = "2011",

month = mar,

doi = "10.1002/etc.434",

language = "English",

volume = "30",

pages = "602--606",

journal = "Environ Toxicol Chem",

issn = "1552-8618",

number = "3",

}

TY - JOUR

T1 - Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources.

AU - Liu, Jian

AU - Pankhurst, Louise J.

AU - Deacon, Lewis J.

AU - Abate, Wondwossen

AU - Hayes, Enda T.

AU - Drew, Gill H.

AU - Longhurst, PJ

AU - Pollard, Simon

AU - Longhurst, J

AU - Tyrrel, Sean F.

AU - Jackson, Simon K.

PY - 2011/3

Y1 - 2011/3

N2 - Because of the lack of effective methodology, the biological effects of environmental endotoxin have not been assessed. Here we have collected and measured airborne endotoxin at different locations around composting sites. Increased endotoxin concentrations were observed close to composting activities and also at nearby boundary areas. Analysis of proinflammatory effects of the environmental endotoxin on interleukin (IL)-8 and IL-6 release from human D562 pharyngeal epithelial and MM6 monocytic cell cultures showed an association between endotoxin level and cytokine induction. The cytokine-inducing effect of bioaerosol extracts was inhibited by polymyxin B, indicating that endotoxin was the cause of cytokine responses we found. The environmental endotoxin was also more active for stimulating cytokines in airway epithelial cells than commercially purified Escherichia coli endotoxin. Our results suggest that these in vitro inflammatory cell models may contribute to the assessment of health impacts of environmental endotoxin.

AB - Because of the lack of effective methodology, the biological effects of environmental endotoxin have not been assessed. Here we have collected and measured airborne endotoxin at different locations around composting sites. Increased endotoxin concentrations were observed close to composting activities and also at nearby boundary areas. Analysis of proinflammatory effects of the environmental endotoxin on interleukin (IL)-8 and IL-6 release from human D562 pharyngeal epithelial and MM6 monocytic cell cultures showed an association between endotoxin level and cytokine induction. The cytokine-inducing effect of bioaerosol extracts was inhibited by polymyxin B, indicating that endotoxin was the cause of cytokine responses we found. The environmental endotoxin was also more active for stimulating cytokines in airway epithelial cells than commercially purified Escherichia coli endotoxin. Our results suggest that these in vitro inflammatory cell models may contribute to the assessment of health impacts of environmental endotoxin.

KW - Air Pollutants

KW - Biodegradation

KW - Environmental

KW - Cell Line

KW - Endotoxins

KW - Humans

KW - Inflammation

KW - Interleukin-6

KW - Interleukin-8

KW - Models

KW - Biological

KW - Respiratory Mucosa

U2 - 10.1002/etc.434

DO - 10.1002/etc.434

M3 - Article

SN - 1552-8618

VL - 30

SP - 602

EP - 606

JO - Environ Toxicol Chem

JF - Environ Toxicol Chem

IS - 3

ER -

Evaluation of inflammatory effects of airborne endotoxin emitted from composting sources.

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this