Abstract
Therapeutic ultrasound (US) is a widely used form of biophysical stimulation that is increasingly applied to promote fracture healing. Transforming growth factor-beta (TGF-beta), which is encoded by three related but different genes, is known to play a major part in bone growth and repair. However, the effects of US on the expression of the TGF-beta genes and the physical acoustic mechanisms involved in initiating changes in gene expression in vitro, are not yet known. The present study demonstrates that US had a differential effect on these TGF-beta isoforms in a human osteoblast cell line, with the highest dose eliciting the most pronounced up-regulation of both TGF-beta1 and TGF-beta3 at 1 hour after treatment and thereafter declining. In contrast, US had no effect on TGF-beta2 expression. Fluid streaming rather than thermal effects or cavitation was found to be the most likely explanation for the gene responses observed in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 70-76 |
| Number of pages | 0 |
| Journal | Eur Cell Mater |
| Volume | 10 |
| Issue number | 0 |
| DOIs | |
| Publication status | Published - 5 Dec 2005 |
Keywords
- Acoustics
- Bone and Bones
- Cell Line
- Tumor
- Gene Expression Regulation
- Humans
- RNA
- Messenger
- Temperature
- Transforming Growth Factor beta1
- Transforming Growth Factor beta2
- Transforming Growth Factor beta3
- Ultrasonics