Effect of Interferon-γ on Membrane Conformation in the Macrophage-Like Cell Line P388D

H. Darmani; J. L. Harwood; S. K. Jackson

doi:10.1089/jir.1993.13.427

Effect of Interferon-γ on Membrane Conformation in the Macrophage-Like Cell Line P388D

H. Darmani, J. L. Harwood, S. K. Jackson^*

^*Corresponding author for this work

School of Biomedical Sciences

Cardiff University

Research output: Contribution to journal › Article › peer-review

Abstract

Interferon-γ (IFN-γ) specifically induced the uptake of the unsaturated fatty acid [14C]linoleic acid into membrane phospholipids of the murine macrophage-like P388D cell lineage, but did not alter the incorporation of the saturated fatty acid [14C]stearic acid. Spin label ESR spectroscopy was used to examine any effects of these IFN-γ-induced changes on membrane fluidity and the results revealed significant increases in plasma membrane fluidity. This alteration in membrane fluidity may have important consequences in the dynamic properties of cellular physiochemical interactions and some of the stimulatory effects of IFN-γ on macrophages might be attributed to its effects on the plasma membrane composition.

Original language	English
Pages (from-to)	427-431
Journal	Journal of Interferon Research
Volume	13
Issue number	6
DOIs	https://doi.org/10.1089/jir.1993.13.427
Publication status	Published - Dec 1993

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title = "Effect of Interferon-γ on Membrane Conformation in the Macrophage-Like Cell Line P388D",

abstract = "Interferon-γ (IFN-γ) specifically induced the uptake of the unsaturated fatty acid [14C]linoleic acid into membrane phospholipids of the murine macrophage-like P388D cell lineage, but did not alter the incorporation of the saturated fatty acid [14C]stearic acid. Spin label ESR spectroscopy was used to examine any effects of these IFN-γ-induced changes on membrane fluidity and the results revealed significant increases in plasma membrane fluidity. This alteration in membrane fluidity may have important consequences in the dynamic properties of cellular physiochemical interactions and some of the stimulatory effects of IFN-γ on macrophages might be attributed to its effects on the plasma membrane composition.",

author = "H. Darmani and Harwood, {J. L.} and Jackson, {S. K.}",

year = "1993",

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AU - Darmani, H.

AU - Harwood, J. L.

AU - Jackson, S. K.

PY - 1993/12

Y1 - 1993/12

N2 - Interferon-γ (IFN-γ) specifically induced the uptake of the unsaturated fatty acid [14C]linoleic acid into membrane phospholipids of the murine macrophage-like P388D cell lineage, but did not alter the incorporation of the saturated fatty acid [14C]stearic acid. Spin label ESR spectroscopy was used to examine any effects of these IFN-γ-induced changes on membrane fluidity and the results revealed significant increases in plasma membrane fluidity. This alteration in membrane fluidity may have important consequences in the dynamic properties of cellular physiochemical interactions and some of the stimulatory effects of IFN-γ on macrophages might be attributed to its effects on the plasma membrane composition.

AB - Interferon-γ (IFN-γ) specifically induced the uptake of the unsaturated fatty acid [14C]linoleic acid into membrane phospholipids of the murine macrophage-like P388D cell lineage, but did not alter the incorporation of the saturated fatty acid [14C]stearic acid. Spin label ESR spectroscopy was used to examine any effects of these IFN-γ-induced changes on membrane fluidity and the results revealed significant increases in plasma membrane fluidity. This alteration in membrane fluidity may have important consequences in the dynamic properties of cellular physiochemical interactions and some of the stimulatory effects of IFN-γ on macrophages might be attributed to its effects on the plasma membrane composition.

U2 - 10.1089/jir.1993.13.427

DO - 10.1089/jir.1993.13.427

M3 - Article

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JO - Journal of Interferon Research

JF - Journal of Interferon Research

IS - 6

ER -

Effect of Interferon-γ on Membrane Conformation in the Macrophage-Like Cell Line P388D

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