Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.

Vian Azzu; Charles Affourtit; Eamon P. Breen; Nadeene Parker; Martin D. Brand

doi:10.1016/j.bbabio.2008.07.001

Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.

Vian Azzu^*
, Charles Affourtit
, Eamon P. Breen
, Nadeene Parker
, Martin D. Brand

^*Corresponding author for this work

Wellcome Trust

Research output: Contribution to journal › Article › peer-review

Abstract

Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.

Original language	English
Pages (from-to)	1378-1383
Number of pages	0
Journal	Biochim Biophys Acta
Volume	1777
Issue number	10
DOIs	https://doi.org/10.1016/j.bbabio.2008.07.001
Publication status	Published - Oct 2008
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Cell Line
Tumor
Glucose
Insulinoma
Ion Channels
Mitochondria
Mitochondrial Proteins
Rats
Uncoupling Protein 2

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10.1016/j.bbabio.2008.07.001

Cite this

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title = "Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.",

abstract = "Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.",

keywords = "Animals, Cell Line, Tumor, Glucose, Insulinoma, Ion Channels, Mitochondria, Mitochondrial Proteins, Rats, Uncoupling Protein 2",

author = "Vian Azzu and Charles Affourtit and Breen, \{Eamon P.\} and Nadeene Parker and Brand, \{Martin D.\}",

year = "2008",

month = oct,

doi = "10.1016/j.bbabio.2008.07.001",

language = "English",

volume = "1777",

pages = "1378--1383",

journal = "Biochim Biophys Acta",

issn = "0006-3002",

number = "10",

}

TY - JOUR

T1 - Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.

AU - Azzu, Vian

AU - Affourtit, Charles

AU - Breen, Eamon P.

AU - Parker, Nadeene

AU - Brand, Martin D.

PY - 2008/10

Y1 - 2008/10

N2 - Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.

AB - Uncoupling protein 2 (UCP2) regulates glucose-stimulated insulin secretion in pancreatic beta-cells. UCP2 content, measured by calibrated immunoblot in INS-1E insulinoma cells (a pancreatic beta-cell model) grown in RPMI medium, and INS-1E mitochondria, was 2.0 ng/million cells (7.9 ng/mg mitochondrial protein). UCP2 content was lower in cells incubated without glutamine and higher in cells incubated with 20 mM glucose, and varied from 1.0-4.4 ng/million cells (2.7-14.5 ng/mg mitochondrial protein). This dynamic response to nutrients was achieved by varied expression rates against a background of a very short UCP2 protein half-life of about 1 h.

KW - Animals

KW - Cell Line

KW - Tumor

KW - Glucose

KW - Insulinoma

KW - Ion Channels

KW - Mitochondria

KW - Mitochondrial Proteins

KW - Rats

KW - Uncoupling Protein 2

U2 - 10.1016/j.bbabio.2008.07.001

DO - 10.1016/j.bbabio.2008.07.001

M3 - Article

SN - 0006-3002

VL - 1777

SP - 1378

EP - 1383

JO - Biochim Biophys Acta

JF - Biochim Biophys Acta

IS - 10

ER -

Dynamic regulation of uncoupling protein 2 content in INS-1E insulinoma cells.

Abstract

UN SDGs

Keywords

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