TY - JOUR
T1 - Dietary Clostridium autoethanogenum protein modulates intestinal absorption, antioxidant status, and immune response in GIFT (Oreochromis niloticus) juveniles
AU - Maulu, Sahya
AU - Hualiang, Liang
AU - Ke, Ji
AU - Ren, Mingchun
AU - Ge, Xianping
AU - Huang, Dongyu
AU - Yu, Heng
N1 - Publisher Copyright:
© 2021 John Wiley & Sons Ltd.
PY - 2021/11
Y1 - 2021/11
N2 - This study investigated the effect of Clostridium autoethanogenum protein (CaP) on intestinal absorption, antioxidant status, and immune response of the genetically improved farmed tilapia (GIFT: Oreochromis niloticus) juveniles. The fish (initial weight 0.7 ± 0.5 g) were fed with diets containing different levels of CaP: 0% (control), 5%, 10%, 15%, and 20% to partially replace soybean meal. After 8 weeks feeding trial, absorption capacity–related sodium/potassium-ATPase transporter (Na+/K+-ATP) was increased significantly by CaP levels; alkaline phosphatase (AKP) improved significantly by 5% and 10% CaP levels; while γ-glutamyl transpeptidase (γ-GT) was not significantly affected compared with the control diet. Malondialdehyde (MDA) content decreased significantly; glutathione (GSH) content and superoxide dismutase (SOD) activity increased significantly; while catalase (CAT) and glutathione peroxidase (GP-x) activities were not significantly affected by dietary CaP compared with the control. The expression of antioxidant-related genes: kelch-like ECH-associated protein 1 (Keap1), nuclear factor erythroid-2-related factor 2 (Nrf2) and glutathione peroxidase (GPx) were significantly down-regulated, up-regulated by 5% and 10%, and not significantly affected by CaP levels compared with the control diet, respectively. The immune-related target of rapamycin (TOR), peptide transporter 1β (Pept 1β), complementary component 3 (C3) and interferon-gamma (IFN-γ) were significantly up-regulated by CaP levels; transforming growth factor-beta (TGF-β) was only significantly up-regulated by 5% and 10% CaP; while interleukin-8 (IL-8) was not significantly affected compared with the control. Therefore, our results show that dietary CaP could enhance the intestinal absorption, antioxidant status, and immune response in GIFT juveniles. However, inclusion levels above 10% could harm the intestinal histology of the fish.
AB - This study investigated the effect of Clostridium autoethanogenum protein (CaP) on intestinal absorption, antioxidant status, and immune response of the genetically improved farmed tilapia (GIFT: Oreochromis niloticus) juveniles. The fish (initial weight 0.7 ± 0.5 g) were fed with diets containing different levels of CaP: 0% (control), 5%, 10%, 15%, and 20% to partially replace soybean meal. After 8 weeks feeding trial, absorption capacity–related sodium/potassium-ATPase transporter (Na+/K+-ATP) was increased significantly by CaP levels; alkaline phosphatase (AKP) improved significantly by 5% and 10% CaP levels; while γ-glutamyl transpeptidase (γ-GT) was not significantly affected compared with the control diet. Malondialdehyde (MDA) content decreased significantly; glutathione (GSH) content and superoxide dismutase (SOD) activity increased significantly; while catalase (CAT) and glutathione peroxidase (GP-x) activities were not significantly affected by dietary CaP compared with the control. The expression of antioxidant-related genes: kelch-like ECH-associated protein 1 (Keap1), nuclear factor erythroid-2-related factor 2 (Nrf2) and glutathione peroxidase (GPx) were significantly down-regulated, up-regulated by 5% and 10%, and not significantly affected by CaP levels compared with the control diet, respectively. The immune-related target of rapamycin (TOR), peptide transporter 1β (Pept 1β), complementary component 3 (C3) and interferon-gamma (IFN-γ) were significantly up-regulated by CaP levels; transforming growth factor-beta (TGF-β) was only significantly up-regulated by 5% and 10% CaP; while interleukin-8 (IL-8) was not significantly affected compared with the control. Therefore, our results show that dietary CaP could enhance the intestinal absorption, antioxidant status, and immune response in GIFT juveniles. However, inclusion levels above 10% could harm the intestinal histology of the fish.
KW - absorption capacity
KW - antioxidant status
KW - bacteria protein
KW - Clostridium autoethanogenum
KW - GIFT juvenile
KW - intestinal health
UR - http://www.scopus.com/inward/record.url?scp=85109382126&partnerID=8YFLogxK
U2 - 10.1111/are.15454
DO - 10.1111/are.15454
M3 - Article
AN - SCOPUS:85109382126
SN - 1355-557X
VL - 52
SP - 5787
EP - 5799
JO - Aquaculture Research
JF - Aquaculture Research
IS - 11
ER -
