@inproceedings{5f62506df64f42479ca4797b1d9a6be6,
title = "Determination of minimal inhibitory concentration of cadmium for Brevibacillus agri C15 and Brevibacillus agri C15 Cdr",
abstract = "Determining resistance by minimal inhibitory concentration (MIC) assay is important in handling bacteria. Here we describe a protocol for identifying the MIC for cadmium (Cd) of Brevibacillus agri C15 and Brevibacillus agri C15 CdR to assist researchers incorrectly confirming the resistance of isolates. In addition, the effects of Cd on the cell-surface structure and morphology, endospore formation, poly-β-hydroxybutyrate and polyphosphate granules were investigated. Its MIC to Cd were 16 ± 0.7 mM and 21± 0.4 mM Cd, respectively. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed no morphological effects of Cd on either of the strains. Both strains have terminal endospores, poly-β-hydroxybutyrate granules and an absence of polyphosphate granules. This projected assay to determine the MIC of cadmium can be applied to the study of new agents with B. agri C15 and B. agri C15 CdR and in laboratory routines for other bacteria.",
keywords = "Brevibacillus, cadmium, endospore, minimum inhibitory concentration, poly-β-hydroxybutyrate granule, polyphosphate granule, SEM, TEM",
author = "Nadia Jebril and Rich Boden and Charlotte Braungardt",
note = "Publisher Copyright: {\textcopyright} 2023 Author(s).; International Conference on Scientific Research and Innovation 2022, ICSRI 2022 ; Conference date: 10-04-2022 Through 11-04-2022",
year = "2023",
month = jun,
day = "26",
doi = "10.1063/5.0150818",
language = "English",
volume = "2820",
series = "AIP Conference Proceedings",
publisher = "American Institute of Physics Inc.",
editor = "Justin Link and Haider Raad and Sarah McGrath and Adawiya Haider and Muayad, {T.A. Mohammed} and Hanady AL-Shmgani and Thaer Al-Jadir",
booktitle = "International Conference on Scientific Research and Innovation 2022, ICSRI 2022",
address = "United States",
}