Detection of genotoxins in the marine environment: adoption and evaluation of an integrated approach using the embryo-larval stages of the marine mussel, Mytilus edulis.

Awadhesh N. Jha*, Victoria V. Cheung, Michael E. Foulkes, Stephen J. Hill, Michael H. Depledge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In genetic ecotoxicology or eco-genotoxicology, there is lack of well-validated systems which could demonstrate the utility of multiple endpoints in environmental quality assessment. For an evaluation of genotoxic potential of heterogeneous marine sediment samples collected from a small fishing harbour in the UK, an in vivo test system using embryo-larval stages of the common mussel, Mytilus edulis was validated against direct and indirect acting reference mutagens. The system appeared to be sensitive and reproducible for cytogenetic endpoints analysed (sister chromatid exchanges (SCEs) and chromosomal aberrations (CAbs)). Following validation and chemical characterisation of the environmental samples, multiple endpoints were measured. Determination of the maximum tolerated dose (MTD) was carried out as a measure to determine cytotoxic effects as a confounding factor for genotoxicity, based on developmental and cytotoxic (in terms of proliferative rate index or PRI) effects. Evaluation of the genotoxic potential of the samples gave a positive response for all the endpoints tested, linking different levels of biological organisation (i.e., chromosomal, cellular and organismal) for the observed effects. The study also emphasises the need for the assessment of the short and long-term impacts of dredge disposal on marine biota by including laboratory-based bioassays and incorporating an integrated approach which could yield as much useful information as possible in overall hazard and risk assessment for aquatic genotoxicity.
Original languageEnglish
Pages (from-to)213-228
Number of pages0
JournalMutat Res
Volume464
Issue number2
DOIs
Publication statusPublished - 24 Jan 2000

Keywords

  • Animals
  • Biological Assay
  • Bivalvia
  • Chromosome Aberrations
  • Dose-Response Relationship
  • Drug
  • Embryo
  • Nonmammalian
  • Environmental Monitoring
  • Geologic Sediments
  • Hydrogen-Ion Concentration
  • Karyotyping
  • Larva
  • Metals
  • Heavy
  • Mutagenicity Tests
  • Mutagens
  • Oxidation-Reduction
  • Oxygen
  • Particle Size
  • Reproducibility of Results
  • Sister Chromatid Exchange
  • Water Pollution
  • Chemical

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