Complete Genome Sequence and Lytic Phase Transcription Profile of a
 Coccolithovirus

William H. Wilson; Declan C. Schroeder; Michael J. Allen; Matthew T.G. Holden; Julian Parkhill; Bart G. Barrell; Carol Churcher; Nancy Hamlin; Karen Mungall; Halina Norbertczak; Michael A. Quail; Claire Price; Ester Rabbinowitsch; Danielle Walker; Marie Craigon; Douglas Roy; Peter Ghazal

doi:10.1126/science.1113109

Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

William H. Wilson^*
, Declan C. Schroeder
, Michael J. Allen
, Matthew T.G. Holden
, Julian Parkhill
, Bart G. Barrell
, Carol Churcher
, Nancy Hamlin
, Karen Mungall
, Halina Norbertczak
, Michael A. Quail
, Claire Price
, Ester Rabbinowitsch
, Danielle Walker
, Marie Craigon
, Douglas Roy
, Peter Ghazal

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

Original language	English
Pages (from-to)	1090-1092
Number of pages	0
Journal	Science
Volume	309
Issue number	5737
DOIs	https://doi.org/10.1126/science.1113109
Publication status	Published - 12 Aug 2005

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Wilson, W. H., Schroeder, D. C., Allen, M. J., Holden, M. T. G., Parkhill, J., Barrell, B. G., Churcher, C., Hamlin, N., Mungall, K., Norbertczak, H., Quail, M. A., Price, C., Rabbinowitsch, E., Walker, D., Craigon, M., Roy, D., & Ghazal, P. (2005). Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus. Science, 309(5737), 1090-1092. https://doi.org/10.1126/science.1113109

@article{b3680c1d9e1a457d8de086240553ff61,

title = "Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus",

abstract = " The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65\% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi . ",

author = "Wilson, \{William H.\} and Schroeder, \{Declan C.\} and Allen, \{Michael J.\} and Holden, \{Matthew T.G.\} and Julian Parkhill and Barrell, \{Bart G.\} and Carol Churcher and Nancy Hamlin and Karen Mungall and Halina Norbertczak and Quail, \{Michael A.\} and Claire Price and Ester Rabbinowitsch and Danielle Walker and Marie Craigon and Douglas Roy and Peter Ghazal",

year = "2005",

month = aug,

day = "12",

doi = "10.1126/science.1113109",

language = "English",

volume = "309",

pages = "1090--1092",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

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}

Wilson, WH, Schroeder, DC, Allen, MJ, Holden, MTG, Parkhill, J, Barrell, BG, Churcher, C, Hamlin, N, Mungall, K, Norbertczak, H, Quail, MA, Price, C, Rabbinowitsch, E, Walker, D, Craigon, M, Roy, D & Ghazal, P 2005, 'Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus', Science, vol. 309, no. 5737, pp. 1090-1092. https://doi.org/10.1126/science.1113109

TY - JOUR

T1 - Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

AU - Wilson, William H.

AU - Schroeder, Declan C.

AU - Allen, Michael J.

AU - Holden, Matthew T.G.

AU - Parkhill, Julian

AU - Barrell, Bart G.

AU - Churcher, Carol

AU - Hamlin, Nancy

AU - Mungall, Karen

AU - Norbertczak, Halina

AU - Quail, Michael A.

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Walker, Danielle

AU - Craigon, Marie

AU - Roy, Douglas

AU - Ghazal, Peter

PY - 2005/8/12

Y1 - 2005/8/12

N2 - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

AB - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

U2 - 10.1126/science.1113109

DO - 10.1126/science.1113109

M3 - Article

SN - 0036-8075

VL - 309

SP - 1090

EP - 1092

JO - Science

JF - Science

IS - 5737

ER -

Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

Abstract

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