Complete Genome Sequence and Lytic Phase Transcription Profile of a
 Coccolithovirus

William H. Wilson; Declan C. Schroeder; Michael J. Allen; Matthew T.G. Holden; Julian Parkhill; Bart G. Barrell; Carol Churcher; Nancy Hamlin; Karen Mungall; Halina Norbertczak; Michael A. Quail; Claire Price; Ester Rabbinowitsch; Danielle Walker; Marie Craigon; Douglas Roy; Peter Ghazal

doi:10.1126/science.1113109

Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

William H. Wilson^*, Declan C. Schroeder, Michael J. Allen, Matthew T.G. Holden, Julian Parkhill, Bart G. Barrell, Carol Churcher, Nancy Hamlin, Karen Mungall, Halina Norbertczak, Michael A. Quail, Claire Price, Ester Rabbinowitsch, Danielle Walker, Marie Craigon, Douglas Roy, Peter Ghazal

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

Original language	English
Pages (from-to)	1090-1092
Number of pages	0
Journal	Science
Volume	309
Issue number	5737
DOIs	https://doi.org/10.1126/science.1113109
Publication status	Published - 12 Aug 2005

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Wilson, W. H., Schroeder, D. C., Allen, M. J., Holden, M. T. G., Parkhill, J., Barrell, B. G., Churcher, C., Hamlin, N., Mungall, K., Norbertczak, H., Quail, M. A., Price, C., Rabbinowitsch, E., Walker, D., Craigon, M., Roy, D., & Ghazal, P. (2005). Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus. Science, 309(5737), 1090-1092. https://doi.org/10.1126/science.1113109

@article{b3680c1d9e1a457d8de086240553ff61,

title = "Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus",

abstract = " The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65\% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi . ",

author = "Wilson, \{William H.\} and Schroeder, \{Declan C.\} and Allen, \{Michael J.\} and Holden, \{Matthew T.G.\} and Julian Parkhill and Barrell, \{Bart G.\} and Carol Churcher and Nancy Hamlin and Karen Mungall and Halina Norbertczak and Quail, \{Michael A.\} and Claire Price and Ester Rabbinowitsch and Danielle Walker and Marie Craigon and Douglas Roy and Peter Ghazal",

year = "2005",

month = aug,

day = "12",

doi = "10.1126/science.1113109",

language = "English",

volume = "309",

pages = "1090--1092",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

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Wilson, WH, Schroeder, DC, Allen, MJ, Holden, MTG, Parkhill, J, Barrell, BG, Churcher, C, Hamlin, N, Mungall, K, Norbertczak, H, Quail, MA, Price, C, Rabbinowitsch, E, Walker, D, Craigon, M, Roy, D & Ghazal, P 2005, 'Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus', Science, vol. 309, no. 5737, pp. 1090-1092. https://doi.org/10.1126/science.1113109

TY - JOUR

T1 - Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

AU - Wilson, William H.

AU - Schroeder, Declan C.

AU - Allen, Michael J.

AU - Holden, Matthew T.G.

AU - Parkhill, Julian

AU - Barrell, Bart G.

AU - Churcher, Carol

AU - Hamlin, Nancy

AU - Mungall, Karen

AU - Norbertczak, Halina

AU - Quail, Michael A.

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Walker, Danielle

AU - Craigon, Marie

AU - Roy, Douglas

AU - Ghazal, Peter

PY - 2005/8/12

Y1 - 2005/8/12

N2 - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

AB - The genus Coccolithovirus is a recently discovered group of viruses that infect the globally important marine calcifying microalga Emiliania huxleyi . Among the 472 predicted genes of the 407,339–base pair genome are a variety of unexpected genes, most notably those involved in biosynthesis of ceramide, a sphingolipid known to induce apoptosis. Uniquely for algal viruses, it also contains six RNA polymerase subunits and a novel promoter, suggesting this virus encodes its own transcription machinery. Microarray transcriptomic analysis reveals that 65% of the predicted virus-encoded genes are expressed during lytic infection of E. huxleyi .

U2 - 10.1126/science.1113109

DO - 10.1126/science.1113109

M3 - Article

SN - 0036-8075

VL - 309

SP - 1090

EP - 1092

JO - Science

JF - Science

IS - 5737

ER -

Complete Genome Sequence and Lytic Phase Transcription Profile of a Coccolithovirus

Abstract

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