Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.

Anthony G. Uren; Miha Pakusch; Christine J. Hawkins; Kirsten L. Puls; David L. Vaux

doi:10.1073/pnas.93.10.4974

Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.

Anthony G. Uren, Miha Pakusch, Christine J. Hawkins, Kirsten L. Puls, David L. Vaux

School of Biomedical Sciences

Royal Melbourne Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death. Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and cowpox virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.

Original language	English
Pages (from-to)	4974-4978
Number of pages	0
Journal	Proceedings of the National Academy of Sciences
Volume	93
Issue number	10
DOIs	https://doi.org/10.1073/pnas.93.10.4974
Publication status	Published - 14 May 1996

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1073/pnas.93.10.4974

Cite this

@article{23dd53e61f8045649200662cc0b2eac2,

title = "Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.",

abstract = "Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death. Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and cowpox virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.",

author = "Uren, {Anthony G.} and Miha Pakusch and Hawkins, {Christine J.} and Puls, {Kirsten L.} and Vaux, {David L.}",

year = "1996",

month = may,

day = "14",

doi = "10.1073/pnas.93.10.4974",

language = "English",

volume = "93",

pages = "4974--4978",

journal = "Proceedings of the National Academy of Sciences",

issn = "0027-8424",

number = "10",

}

Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors. / Uren, Anthony G.; Pakusch, Miha; Hawkins, Christine J. et al.
In: Proceedings of the National Academy of Sciences, Vol. 93, No. 10, 14.05.1996, p. 4974-4978.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.

AU - Uren, Anthony G.

AU - Pakusch, Miha

AU - Hawkins, Christine J.

AU - Puls, Kirsten L.

AU - Vaux, David L.

PY - 1996/5/14

Y1 - 1996/5/14

N2 - Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death. Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and cowpox virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.

AB - Baculovirus inhibitors of apoptosis (IAPs) act in insect cells to prevent cell death. Here we describe three mammalian homologs of IAP, MIHA, MIHB, and MIHC, and a Drosophila IAP homolog, DIHA. Each protein bears three baculovirus IAP repeats and an N-terminal ring finger motif. Apoptosis mediated by interleukin 1beta converting enzyme (ICE), which can be inhibited by Orgyia pseudotsugata nuclear polyhedrosis virus IAP (OpIAP) and cowpox virus crmA, was also inhibited by MIHA and MIHB. As MIHB and MIHC were able to bind to the tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 in yeast two-hybrid assays, these results suggest that IAP proteins that inhibit apoptosis may do so by regulating signals required for activation of ICE-like proteases.

U2 - 10.1073/pnas.93.10.4974

DO - 10.1073/pnas.93.10.4974

M3 - Article

SN - 0027-8424

VL - 93

SP - 4974

EP - 4978

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

IS - 10

ER -

Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors.

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this