Characterizing the Pathogenic, Genomic, and Chemical Traits of
 Aspergillus fischeri
 , a Close Relative of the Major Human Fungal Pathogen
 Aspergillus fumigatus

Matthew E. Mead; Sonja L. Knowles; Huzefa A. Raja; Sarah R. Beattie; Caitlin H. Kowalski; Jacob L. Steenwyk; Lilian P. Silva; Jessica Chiaratto; Laure N.A. Ries; Gustavo H. Goldman; Robert A. Cramer; Nicholas H. Oberlies; Antonis Rokas

doi:10.1128/msphere.00018-19

Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus

Matthew E. Mead, Sonja L. Knowles, Huzefa A. Raja, Sarah R. Beattie, Caitlin H. Kowalski, Jacob L. Steenwyk, Lilian P. Silva, Jessica Chiaratto, Laure N.A. Ries, Gustavo H. Goldman, Robert A. Cramer, Nicholas H. Oberlies, Antonis Rokas^*

^*Corresponding author for this work

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Aspergillus fumigatus is the primary cause of aspergillosis, a devastating ensemble of diseases associated with severe morbidity and mortality worldwide. A. fischeri is a close relative of A. fumigatus but is not generally observed to cause human disease. To gain insights into the underlying causes of this remarkable difference in pathogenicity, we compared two representative strains (one from each species) for a range of pathogenesis-relevant biological and chemical characteristics. We found that disease progression in multiple A. fischeri mouse models was slower and caused less mortality than A. fumigatus . Remarkably, the observed differences between A. fischeri and A. fumigatus strains examined here closely resembled those previously described for two commonly studied A. fumigatus strains, AF293 and CEA10. A. fischeri and A. fumigatus exhibited different growth profiles when placed in a range of stress-inducing conditions encountered during infection, such as low levels of oxygen and the presence of chemicals that induce the production of reactive oxygen species. We also found that the vast majority of A. fumigatus genes known to be involved in virulence are conserved in A. fischeri , whereas the two species differ significantly in their secondary metabolic pathways. These similarities and differences that we report here are the first step toward understanding the evolutionary origin of a major fungal pathogen.

Original language	English
Number of pages	0
Journal	mSphere
Volume	4
Issue number	1
DOIs	https://doi.org/10.1128/msphere.00018-19
Publication status	Published - 27 Feb 2019

Access to Document

10.1128/msphere.00018-19

Cite this

Mead, M. E., Knowles, S. L., Raja, H. A., Beattie, S. R., Kowalski, C. H., Steenwyk, J. L., Silva, L. P., Chiaratto, J., Ries, L. N. A., Goldman, G. H., Cramer, R. A., Oberlies, N. H., & Rokas, A. (2019). Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus. mSphere, 4(1). https://doi.org/10.1128/msphere.00018-19

@article{5cf7e785ddd043368623fe566a649e24,

title = "Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus",

abstract = " Aspergillus fumigatus is the primary cause of aspergillosis, a devastating ensemble of diseases associated with severe morbidity and mortality worldwide. A. fischeri is a close relative of A. fumigatus but is not generally observed to cause human disease. To gain insights into the underlying causes of this remarkable difference in pathogenicity, we compared two representative strains (one from each species) for a range of pathogenesis-relevant biological and chemical characteristics. We found that disease progression in multiple A. fischeri mouse models was slower and caused less mortality than A. fumigatus . Remarkably, the observed differences between A. fischeri and A. fumigatus strains examined here closely resembled those previously described for two commonly studied A. fumigatus strains, AF293 and CEA10. A. fischeri and A. fumigatus exhibited different growth profiles when placed in a range of stress-inducing conditions encountered during infection, such as low levels of oxygen and the presence of chemicals that induce the production of reactive oxygen species. We also found that the vast majority of A. fumigatus genes known to be involved in virulence are conserved in A. fischeri , whereas the two species differ significantly in their secondary metabolic pathways. These similarities and differences that we report here are the first step toward understanding the evolutionary origin of a major fungal pathogen. ",

author = "Mead, {Matthew E.} and Knowles, {Sonja L.} and Raja, {Huzefa A.} and Beattie, {Sarah R.} and Kowalski, {Caitlin H.} and Steenwyk, {Jacob L.} and Silva, {Lilian P.} and Jessica Chiaratto and Ries, {Laure N.A.} and Goldman, {Gustavo H.} and Cramer, {Robert A.} and Oberlies, {Nicholas H.} and Antonis Rokas",

year = "2019",

month = feb,

day = "27",

doi = "10.1128/msphere.00018-19",

language = "English",

volume = "4",

journal = "mSphere",

issn = "2379-5042",

publisher = "American Society for Microbiology",

number = "1",

}

Mead, ME, Knowles, SL, Raja, HA, Beattie, SR, Kowalski, CH, Steenwyk, JL, Silva, LP, Chiaratto, J, Ries, LNA, Goldman, GH, Cramer, RA, Oberlies, NH & Rokas, A 2019, 'Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus', mSphere, vol. 4, no. 1. https://doi.org/10.1128/msphere.00018-19

TY - JOUR

T1 - Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus

AU - Mead, Matthew E.

AU - Knowles, Sonja L.

AU - Raja, Huzefa A.

AU - Beattie, Sarah R.

AU - Kowalski, Caitlin H.

AU - Steenwyk, Jacob L.

AU - Silva, Lilian P.

AU - Chiaratto, Jessica

AU - Ries, Laure N.A.

AU - Goldman, Gustavo H.

AU - Cramer, Robert A.

AU - Oberlies, Nicholas H.

AU - Rokas, Antonis

PY - 2019/2/27

Y1 - 2019/2/27

N2 - Aspergillus fumigatus is the primary cause of aspergillosis, a devastating ensemble of diseases associated with severe morbidity and mortality worldwide. A. fischeri is a close relative of A. fumigatus but is not generally observed to cause human disease. To gain insights into the underlying causes of this remarkable difference in pathogenicity, we compared two representative strains (one from each species) for a range of pathogenesis-relevant biological and chemical characteristics. We found that disease progression in multiple A. fischeri mouse models was slower and caused less mortality than A. fumigatus . Remarkably, the observed differences between A. fischeri and A. fumigatus strains examined here closely resembled those previously described for two commonly studied A. fumigatus strains, AF293 and CEA10. A. fischeri and A. fumigatus exhibited different growth profiles when placed in a range of stress-inducing conditions encountered during infection, such as low levels of oxygen and the presence of chemicals that induce the production of reactive oxygen species. We also found that the vast majority of A. fumigatus genes known to be involved in virulence are conserved in A. fischeri , whereas the two species differ significantly in their secondary metabolic pathways. These similarities and differences that we report here are the first step toward understanding the evolutionary origin of a major fungal pathogen.

AB - Aspergillus fumigatus is the primary cause of aspergillosis, a devastating ensemble of diseases associated with severe morbidity and mortality worldwide. A. fischeri is a close relative of A. fumigatus but is not generally observed to cause human disease. To gain insights into the underlying causes of this remarkable difference in pathogenicity, we compared two representative strains (one from each species) for a range of pathogenesis-relevant biological and chemical characteristics. We found that disease progression in multiple A. fischeri mouse models was slower and caused less mortality than A. fumigatus . Remarkably, the observed differences between A. fischeri and A. fumigatus strains examined here closely resembled those previously described for two commonly studied A. fumigatus strains, AF293 and CEA10. A. fischeri and A. fumigatus exhibited different growth profiles when placed in a range of stress-inducing conditions encountered during infection, such as low levels of oxygen and the presence of chemicals that induce the production of reactive oxygen species. We also found that the vast majority of A. fumigatus genes known to be involved in virulence are conserved in A. fischeri , whereas the two species differ significantly in their secondary metabolic pathways. These similarities and differences that we report here are the first step toward understanding the evolutionary origin of a major fungal pathogen.

U2 - 10.1128/msphere.00018-19

DO - 10.1128/msphere.00018-19

M3 - Article

SN - 2379-5042

VL - 4

JO - mSphere

JF - mSphere

IS - 1

ER -

Characterizing the Pathogenic, Genomic, and Chemical Traits of Aspergillus fischeri , a Close Relative of the Major Human Fungal Pathogen Aspergillus fumigatus

Abstract

Access to Document

Fingerprint

Cite this