Characterization of NAD uptake in mammalian cells.

Richard A. Billington; Cristina Travelli; Emanuela Ercolano; Ubaldina Galli; Cintia Blasi Roman; Ambra A. Grolla; Pier Luigi Canonico; Fabrizio Condorelli; Armando A. Genazzani

doi:10.1074/jbc.M706204200

Characterization of NAD uptake in mammalian cells.

Richard A. Billington, Cristina Travelli, Emanuela Ercolano, Ubaldina Galli, Cintia Blasi Roman, Ambra A. Grolla, Pier Luigi Canonico, Fabrizio Condorelli, Armando A. Genazzani

University of Eastern Piedmont

Research output: Contribution to journal › Article › peer-review

Abstract

Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.

Original language	English
Pages (from-to)	6367-6374
Number of pages	0
Journal	J Biol Chem
Volume	283
Issue number	10
DOIs	https://doi.org/10.1074/jbc.M706204200
Publication status	Published - 7 Mar 2008

Keywords

Acrylamides
Animals
Biological Transport
Cell Death
Dose-Response Relationship
Drug
HeLa Cells
Homeostasis
Humans
K562 Cells
Mice
NAD
NADP
NIH 3T3 Cells
Piperidines
Signal Transduction

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10.1074/jbc.M706204200

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title = "Characterization of NAD uptake in mammalian cells.",

abstract = "Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.",

keywords = "Acrylamides, Animals, Biological Transport, Cell Death, Dose-Response Relationship, Drug, HeLa Cells, Homeostasis, Humans, K562 Cells, Mice, NAD, NADP, NIH 3T3 Cells, Piperidines, Signal Transduction",

author = "Billington, {Richard A.} and Cristina Travelli and Emanuela Ercolano and Ubaldina Galli and Roman, {Cintia Blasi} and Grolla, {Ambra A.} and Canonico, {Pier Luigi} and Fabrizio Condorelli and Genazzani, {Armando A.}",

year = "2008",

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doi = "10.1074/jbc.M706204200",

language = "English",

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journal = "J Biol Chem",

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T1 - Characterization of NAD uptake in mammalian cells.

AU - Billington, Richard A.

AU - Travelli, Cristina

AU - Ercolano, Emanuela

AU - Galli, Ubaldina

AU - Roman, Cintia Blasi

AU - Grolla, Ambra A.

AU - Canonico, Pier Luigi

AU - Condorelli, Fabrizio

AU - Genazzani, Armando A.

PY - 2008/3/7

Y1 - 2008/3/7

N2 - Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.

AB - Recent evidence has shown that NAD(P) plays a variety of roles in cell-signaling processes. Surprisingly, the presence of NAD(P) utilizing ectoenzymes suggests that NAD(P) is present extracellularly. Indeed, nanomolar concentrations of NAD have been found in plasma and other body fluids. Although very high concentrations of NAD have been shown to enter cells, it is not known whether lower, more physiological concentrations are able to be taken up. Here we show that two mammalian cell types are able to transport low NAD concentrations effectively. Furthermore, extracellular application of NAD was able to counteract FK866-induced cell death and restore intracellular NAD(P) levels. We propose that NAD uptake may play a role in physiological NAD homeostasis.

KW - Acrylamides

KW - Animals

KW - Biological Transport

KW - Cell Death

KW - Dose-Response Relationship

KW - Drug

KW - HeLa Cells

KW - Homeostasis

KW - Humans

KW - K562 Cells

KW - Mice

KW - NAD

KW - NADP

KW - NIH 3T3 Cells

KW - Piperidines

KW - Signal Transduction

U2 - 10.1074/jbc.M706204200

DO - 10.1074/jbc.M706204200

M3 - Article

SN - 0021-9258

VL - 283

SP - 6367

EP - 6374

JO - J Biol Chem

JF - J Biol Chem

IS - 10

ER -

Characterization of NAD uptake in mammalian cells.

Abstract

Keywords

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