beta-Neuregulin and autocrine mediated survival of Schwann cells requires activity of Ets family transcription factors.

David B. Parkinson; Katrin Langner; Soheila Sharghi Namini; Kristjan R. Jessen; Rhona Mirsky

doi:10.1006/mcne.2002.1109

beta-Neuregulin and autocrine mediated survival of Schwann cells requires activity of Ets family transcription factors.

David B. Parkinson^*, Katrin Langner, Soheila Sharghi Namini, Kristjan R. Jessen, Rhona Mirsky

^*Corresponding author for this work

Peninsula Medical School

University College London

Research output: Contribution to journal › Article › peer-review

Abstract

Members of the Ets transcription factor family function in many biological processes. We show the presence of Ets transcription factors, most prominently Net, in neonatal rat Schwann cells, and demonstrate Ets-dependent transcription under conditions where the cells are exposed to autocrine signals or autocrine signals plus beta-neuregulin. Using the potent MAPK kinase inhibitor U0126 we also confirm that the MAP kinase pathway, an activator of Ets transcription, is involved in beta-neuregulin mediated Schwann cell survival. Furthermore, we find that expression of dominant negative Ets1 (N70-Ets1) inhibits both the beta-neuregulin and autocrine survival of Schwann cells. In contrast, the survival of Schwann cells mediated by lysophosphatidic acid (LPA) is unaffected by expression of a dominant negative Ets molecule. These data demonstrate that distinct autocrine and beta-neuregulin survival signals converge in their requirement for Ets dependent transcription in Schwann cell survival.

Original language	English
Pages (from-to)	154-167
Number of pages	0
Journal	Mol Cell Neurosci
Volume	20
Issue number	1
DOIs	https://doi.org/10.1006/mcne.2002.1109
Publication status	Published - May 2002

Keywords

Animals
Newborn
Apoptosis
Autocrine Communication
Basic Helix-Loop-Helix Transcription Factors
Cell Survival
Cells
Cultured
DNA-Binding Proteins
Drosophila Proteins
Enzyme Inhibitors
GA-Binding Protein Transcription Factor
Gene Expression Regulation
Developmental
Lysophospholipids
MAP Kinase Signaling System
Nerve Regeneration
Neuregulin-1
Peripheral Nerves
Proto-Oncogene Protein c-ets-1
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-ets
Rats
Sprague-Dawley
Repressor Proteins
Schwann Cells
Sciatic Nerve
Stem Cells
Transcription Factors
Transcription
Genetic

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title = "beta-Neuregulin and autocrine mediated survival of Schwann cells requires activity of Ets family transcription factors.",

abstract = "Members of the Ets transcription factor family function in many biological processes. We show the presence of Ets transcription factors, most prominently Net, in neonatal rat Schwann cells, and demonstrate Ets-dependent transcription under conditions where the cells are exposed to autocrine signals or autocrine signals plus beta-neuregulin. Using the potent MAPK kinase inhibitor U0126 we also confirm that the MAP kinase pathway, an activator of Ets transcription, is involved in beta-neuregulin mediated Schwann cell survival. Furthermore, we find that expression of dominant negative Ets1 (N70-Ets1) inhibits both the beta-neuregulin and autocrine survival of Schwann cells. In contrast, the survival of Schwann cells mediated by lysophosphatidic acid (LPA) is unaffected by expression of a dominant negative Ets molecule. These data demonstrate that distinct autocrine and beta-neuregulin survival signals converge in their requirement for Ets dependent transcription in Schwann cell survival.",

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author = "Parkinson, {David B.} and Katrin Langner and Namini, {Soheila Sharghi} and Jessen, {Kristjan R.} and Rhona Mirsky",

year = "2002",

month = may,

doi = "10.1006/mcne.2002.1109",

language = "English",

volume = "20",

pages = "154--167",

journal = "Mol Cell Neurosci",

issn = "1044-7431",

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AU - Parkinson, David B.

AU - Langner, Katrin

AU - Namini, Soheila Sharghi

AU - Jessen, Kristjan R.

AU - Mirsky, Rhona

PY - 2002/5

Y1 - 2002/5

N2 - Members of the Ets transcription factor family function in many biological processes. We show the presence of Ets transcription factors, most prominently Net, in neonatal rat Schwann cells, and demonstrate Ets-dependent transcription under conditions where the cells are exposed to autocrine signals or autocrine signals plus beta-neuregulin. Using the potent MAPK kinase inhibitor U0126 we also confirm that the MAP kinase pathway, an activator of Ets transcription, is involved in beta-neuregulin mediated Schwann cell survival. Furthermore, we find that expression of dominant negative Ets1 (N70-Ets1) inhibits both the beta-neuregulin and autocrine survival of Schwann cells. In contrast, the survival of Schwann cells mediated by lysophosphatidic acid (LPA) is unaffected by expression of a dominant negative Ets molecule. These data demonstrate that distinct autocrine and beta-neuregulin survival signals converge in their requirement for Ets dependent transcription in Schwann cell survival.

AB - Members of the Ets transcription factor family function in many biological processes. We show the presence of Ets transcription factors, most prominently Net, in neonatal rat Schwann cells, and demonstrate Ets-dependent transcription under conditions where the cells are exposed to autocrine signals or autocrine signals plus beta-neuregulin. Using the potent MAPK kinase inhibitor U0126 we also confirm that the MAP kinase pathway, an activator of Ets transcription, is involved in beta-neuregulin mediated Schwann cell survival. Furthermore, we find that expression of dominant negative Ets1 (N70-Ets1) inhibits both the beta-neuregulin and autocrine survival of Schwann cells. In contrast, the survival of Schwann cells mediated by lysophosphatidic acid (LPA) is unaffected by expression of a dominant negative Ets molecule. These data demonstrate that distinct autocrine and beta-neuregulin survival signals converge in their requirement for Ets dependent transcription in Schwann cell survival.

KW - Animals

KW - Newborn

KW - Apoptosis

KW - Autocrine Communication

KW - Basic Helix-Loop-Helix Transcription Factors

KW - Cell Survival

KW - Cells

KW - Cultured

KW - DNA-Binding Proteins

KW - Drosophila Proteins

KW - Enzyme Inhibitors

KW - GA-Binding Protein Transcription Factor

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KW - Developmental

KW - Lysophospholipids

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KW - Proto-Oncogene Protein c-ets-1

KW - Proto-Oncogene Proteins

KW - Proto-Oncogene Proteins c-ets

KW - Rats

KW - Sprague-Dawley

KW - Repressor Proteins

KW - Schwann Cells

KW - Sciatic Nerve

KW - Stem Cells

KW - Transcription Factors

KW - Transcription

KW - Genetic

U2 - 10.1006/mcne.2002.1109

DO - 10.1006/mcne.2002.1109

M3 - Article

SN - 1044-7431

VL - 20

SP - 154

EP - 167

JO - Mol Cell Neurosci

JF - Mol Cell Neurosci

IS - 1

ER -

beta-Neuregulin and autocrine mediated survival of Schwann cells requires activity of Ets family transcription factors.

Abstract

Keywords

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