Autochthonous probiotic bacteria modulate intestinal microbiota of Pirarucu, Arapaima gigas

Vale Pereira G do, Scheila Anelise Pereira, Arno Soares, Jose Luiz Pedreira Mouriño, Daniel Merrifield

Research output: Contribution to journalArticlepeer-review

Abstract

<jats:title>Abstract</jats:title><jats:p>This study aimed to investigate the ability of two probiotic strains (<jats:italic>Lactococcus lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> and <jats:italic>Enterococcus faecium</jats:italic>) to colonize <jats:italic>Arapaima gigas</jats:italic> intestines and their competence in modulating gut microbiota, morphology, and immunological status. A total of 135 <jats:italic>A. gigas</jats:italic> (58.86 ± 10.25 g) were divided into three treatments: (a) fish fed a diet containing <jats:italic>L. lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> at 1 × 10<jats:sup>8</jats:sup> CFU<jats:sup>−1</jats:sup>, (b) fish fed a diet containing <jats:italic>E. faecium</jats:italic> at 1 × 10<jats:sup>8</jats:sup> CFU<jats:sup>−1</jats:sup>, and (c) fish fed a diet nonsupplemented with probiotic. After 21 days, 12 fish per treatment were sampled. Blood and posterior intestine were sampled for further analysis. High‐throughput sequencing results demonstrated that the relative abundance of <jats:italic>Cetobacterium</jats:italic> genus was high in all treatments. The number of thrombocytes increased in both groups fed the probiotic, and eosinophils were higher in fish fed <jats:italic>E. faecium</jats:italic>. Serum antimicrobial activity of fish fed <jats:italic>L. lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> was higher when compared with the other two treatments. In conclusion, both strains could populate the intestinal mucosa of <jats:italic>A. gigas</jats:italic> and could be used as probiotics in pirarucu production as they can influence hematoimmunological parameters and decrease the abundance of pathogenic bacteria in the gut of pirarucu within 21 days of feeding.</jats:p>
Original languageEnglish
Pages (from-to)1152-1167
Number of pages0
JournalJournal of the World Aquaculture Society
Volume50
Issue number6
Early online date29 Jul 2019
DOIs
Publication statusPublished - Dec 2019

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