Abstract
<jats:title>Abstract</jats:title><jats:p>This study aimed to investigate the ability of two probiotic strains (<jats:italic>Lactococcus lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> and <jats:italic>Enterococcus faecium</jats:italic>) to colonize <jats:italic>Arapaima gigas</jats:italic> intestines and their competence in modulating gut microbiota, morphology, and immunological status. A total of 135 <jats:italic>A. gigas</jats:italic> (58.86 ± 10.25 g) were divided into three treatments: (a) fish fed a diet containing <jats:italic>L. lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> at 1 × 10<jats:sup>8</jats:sup> CFU<jats:sup>−1</jats:sup>, (b) fish fed a diet containing <jats:italic>E. faecium</jats:italic> at 1 × 10<jats:sup>8</jats:sup> CFU<jats:sup>−1</jats:sup>, and (c) fish fed a diet nonsupplemented with probiotic. After 21 days, 12 fish per treatment were sampled. Blood and posterior intestine were sampled for further analysis. High‐throughput sequencing results demonstrated that the relative abundance of <jats:italic>Cetobacterium</jats:italic> genus was high in all treatments. The number of thrombocytes increased in both groups fed the probiotic, and eosinophils were higher in fish fed <jats:italic>E. faecium</jats:italic>. Serum antimicrobial activity of fish fed <jats:italic>L. lactis</jats:italic> subsp. <jats:italic>lactis</jats:italic> was higher when compared with the other two treatments. In conclusion, both strains could populate the intestinal mucosa of <jats:italic>A. gigas</jats:italic> and could be used as probiotics in pirarucu production as they can influence hematoimmunological parameters and decrease the abundance of pathogenic bacteria in the gut of pirarucu within 21 days of feeding.</jats:p>
Original language | English |
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Pages (from-to) | 1152-1167 |
Number of pages | 0 |
Journal | Journal of the World Aquaculture Society |
Volume | 50 |
Issue number | 6 |
Early online date | 29 Jul 2019 |
DOIs | |
Publication status | Published - Dec 2019 |