Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.

H. C. Reinardy; E. Skippins; T. B. Henry; A. N. Jha

doi:10.1111/jfb.12042

Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.

H. C. Reinardy, E. Skippins, T. B. Henry^*, A. N. Jha

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

Original language	English
Pages (from-to)	1074-1081
Number of pages	0
Journal	J Fish Biol
Volume	82
Issue number	3
DOIs	https://doi.org/10.1111/jfb.12042
Publication status	Published - Mar 2013

Keywords

Animals
DNA Damage
Hydrogen Peroxide
Male
Sperm Count
Spermatozoa
Zebrafish

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10.1111/jfb.12042

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title = "Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.",

abstract = "Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.",

keywords = "Animals, DNA Damage, Hydrogen Peroxide, Male, Sperm Count, Spermatozoa, Zebrafish",

author = "Reinardy, {H. C.} and E. Skippins and Henry, {T. B.} and Jha, {A. N.}",

year = "2013",

month = mar,

doi = "10.1111/jfb.12042",

language = "English",

volume = "82",

pages = "1074--1081",

journal = "J Fish Biol",

issn = "1095-8649",

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T1 - Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.

AU - Reinardy, H. C.

AU - Skippins, E.

AU - Henry, T. B.

AU - Jha, A. N.

PY - 2013/3

Y1 - 2013/3

N2 - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

AB - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2 O2 , 0-200 µM), and in vitro exposure of sperm to 200 µM H2 O2 produced 88·7 ± 3·9% tail DNA compared to unexposed controls [12 ± 0·7% tail DNA (mean ± s.e., n = 3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4 days, respectively.

KW - Animals

KW - DNA Damage

KW - Hydrogen Peroxide

KW - Male

KW - Sperm Count

KW - Spermatozoa

KW - Zebrafish

U2 - 10.1111/jfb.12042

DO - 10.1111/jfb.12042

M3 - Article

SN - 1095-8649

VL - 82

SP - 1074

EP - 1081

JO - J Fish Biol

JF - J Fish Biol

IS - 3

ER -

Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio.

Abstract

Keywords

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