Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods

L Vezzulli; C Oliveri; A Borello; L Gregory; I Kimirei; M Brunetta; R Stern; S Coco; L Longo; E Taviani; A Santos; J Martinez-Urtaza; WH Wilson; RR Colwell; C Pruzzo; P-D Plisnier

doi:10.1038/s43705-021-00023-1

Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods

L Vezzulli, C Oliveri, A Borello, L Gregory, I Kimirei, M Brunetta, R Stern, S Coco, L Longo, E Taviani, A Santos, J Martinez-Urtaza, WH Wilson, RR Colwell, C Pruzzo, P-D Plisnier

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae.

Original language	English
Number of pages	0
Journal	ISME Communications
Volume	1
Issue number	1
Early online date	7 Jun 2021
DOIs	https://doi.org/10.1038/s43705-021-00023-1
Publication status	Published - 1 Dec 2021

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Vezzulli, L., Oliveri, C., Borello, A., Gregory, L., Kimirei, I., Brunetta, M., Stern, R., Coco, S., Longo, L., Taviani, E., Santos, A., Martinez-Urtaza, J., Wilson, WH., Colwell, RR., Pruzzo, C., & Plisnier, P.-D. (2021). Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods. ISME Communications, 1(1). https://doi.org/10.1038/s43705-021-00023-1

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title = "Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods",

abstract = "Abstract The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae.",

author = "L Vezzulli and C Oliveri and A Borello and L Gregory and I Kimirei and M Brunetta and R Stern and S Coco and L Longo and E Taviani and A Santos and J Martinez-Urtaza and WH Wilson and RR Colwell and C Pruzzo and P-D Plisnier",

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Vezzulli, L, Oliveri, C, Borello, A, Gregory, L, Kimirei, I, Brunetta, M, Stern, R, Coco, S, Longo, L, Taviani, E, Santos, A, Martinez-Urtaza, J, Wilson, WH, Colwell, RR, Pruzzo, C & Plisnier, P-D 2021, 'Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods', ISME Communications, vol. 1, no. 1. https://doi.org/10.1038/s43705-021-00023-1

TY - JOUR

T1 - Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods

AU - Vezzulli, L

AU - Oliveri, C

AU - Borello, A

AU - Gregory, L

AU - Kimirei, I

AU - Brunetta, M

AU - Stern, R

AU - Coco, S

AU - Longo, L

AU - Taviani, E

AU - Santos, A

AU - Martinez-Urtaza, J

AU - Wilson, WH

AU - Colwell, RR

AU - Pruzzo, C

AU - Plisnier, P-D

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Abstract The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae.

AB - Abstract The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae.

U2 - 10.1038/s43705-021-00023-1

DO - 10.1038/s43705-021-00023-1

M3 - Article

SN - 2730-6151

VL - 1

JO - ISME Communications

JF - ISME Communications

IS - 1

ER -

Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods

Abstract

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