TY - JOUR
T1 - An organotypic oral mucosal infection model to study host-pathogen interactions
AU - Salih, Vehid M.
AU - Gould, Samantha J.
AU - Foey, Andrew D.
PY - 2023
Y1 - 2023
N2 - Early in vitro oral mucosal infection models (OMMs) failed to consider the suitability of the model environment to represent the host immune response. Denture stomatitis (DS) is mediated by Candida albicans, but the role of Staphylococcus aureus remains uncertain. A collagen hydrogel-based OMM containing HaCaT and HGF cell types was developed, characterised and employed to study of tissue invasion and pro-inflammatory cytokine production in response to pathogens. Models formed a robust epithelium. Despite their inflammatory baseline, 24-h infection with C. albicans, and/or S. aureus led to tissue invasion, and significantly upregulated IL-6 and IL-8 production by OMMs when compared to the unstimulated control. No significant difference in IL-6 or IL-8 production by OMMs was observed between single and dual infections. These attributes indicate that this newly developed OMM is suitable for the study of DS and could be implemented for the wider study of oral infection.
AB - Early in vitro oral mucosal infection models (OMMs) failed to consider the suitability of the model environment to represent the host immune response. Denture stomatitis (DS) is mediated by Candida albicans, but the role of Staphylococcus aureus remains uncertain. A collagen hydrogel-based OMM containing HaCaT and HGF cell types was developed, characterised and employed to study of tissue invasion and pro-inflammatory cytokine production in response to pathogens. Models formed a robust epithelium. Despite their inflammatory baseline, 24-h infection with C. albicans, and/or S. aureus led to tissue invasion, and significantly upregulated IL-6 and IL-8 production by OMMs when compared to the unstimulated control. No significant difference in IL-6 or IL-8 production by OMMs was observed between single and dual infections. These attributes indicate that this newly developed OMM is suitable for the study of DS and could be implemented for the wider study of oral infection.
UR - https://doi.org/10.1177/20417314231197310
UR - https://pearl.plymouth.ac.uk/context/bhs-research/article/1056/viewcontent/An_20organotypic_20oral_20mucosal_20infection_20model_20to_20study_20host_pathogen_20interactions.pdf
U2 - 10.1177/20417314231197310
DO - 10.1177/20417314231197310
M3 - Article
SN - 2041-7314
VL - 14
JO - Journal of Tissue Engineering
JF - Journal of Tissue Engineering
IS - 0
ER -