Advanced models to study liver sinusoids

Sara Campinoti; Elena Palma; Shilpa Chokshi; Luca Urbani

doi:10.1016/B978-0-323-95262-0.00007-3

Advanced models to study liver sinusoids

Sara Campinoti, Elena Palma, Shilpa Chokshi, Luca Urbani

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Liver sinusoids are microfluidic systems formed by endothelial cells (LSECs) and other resident cells that constitute liver-specific microvasculature. LSECs line the interface between the circulatory system and hepatocytes, acting as gatekeepers of liver homeostasis and immunity. Several liver diseases are associated with the impairment of hepatic sinusoid function. In particular, although extensive studies have attested to the role of LSECs in liver pathophysiology, there are still many questions that remain unanswered regarding the mechanisms mediated by LSECs in liver homeostasis and liver diseases. This is mainly due to the complexity of liver sinusoid biology, which is hard to reproduce in hepatic models. Recently, different advanced models able to reproduce or mimic sinusoid-like structures have been described. The aim of this chapter is to summarize innovative in vitro, ex vivo, and in vivo models that enable the study of sinusoid biology and their key roles in disease development, progression, and response to drugs.

Original language	English
Title of host publication	Sinusoidal Cells in Liver Diseases
Subtitle of host publication	Role in their Pathophysiology, Diagnosis, and Treatment
Publisher	Elsevier Science Ltd.
Pages	135-160
Number of pages	26
ISBN (Electronic)	9780323952620
ISBN (Print)	9780323952637
DOIs	https://doi.org/10.1016/B978-0-323-95262-0.00007-3
Publication status	Published - 1 Jan 2024
Externally published	Yes

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Biochemistry,Genetics and Molecular Biology

Keywords

Animal models
Biology experimental methods
Biomaterials
Biomedical engineering
Bioprinting
Bioreactors
Cell biology
Cellular process
Chronic liver disease
Decellularization
Decellularized scaffolds
Extracellular matrix
Hydrogels
In vitro models
iPSC
Liver sinusoidal endothelial cells
Liver-on-a-chip
Materials in biotechnology
Multicellular models
Organoids
Precision-cut liver slices
Spheroids
Stem cells research
Tissue engineering

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10.1016/B978-0-323-95262-0.00007-3

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title = "Advanced models to study liver sinusoids",

abstract = "Liver sinusoids are microfluidic systems formed by endothelial cells (LSECs) and other resident cells that constitute liver-specific microvasculature. LSECs line the interface between the circulatory system and hepatocytes, acting as gatekeepers of liver homeostasis and immunity. Several liver diseases are associated with the impairment of hepatic sinusoid function. In particular, although extensive studies have attested to the role of LSECs in liver pathophysiology, there are still many questions that remain unanswered regarding the mechanisms mediated by LSECs in liver homeostasis and liver diseases. This is mainly due to the complexity of liver sinusoid biology, which is hard to reproduce in hepatic models. Recently, different advanced models able to reproduce or mimic sinusoid-like structures have been described. The aim of this chapter is to summarize innovative in vitro, ex vivo, and in vivo models that enable the study of sinusoid biology and their key roles in disease development, progression, and response to drugs.",

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author = "Sara Campinoti and Elena Palma and Shilpa Chokshi and Luca Urbani",

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AU - Campinoti, Sara

AU - Palma, Elena

AU - Chokshi, Shilpa

AU - Urbani, Luca

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N2 - Liver sinusoids are microfluidic systems formed by endothelial cells (LSECs) and other resident cells that constitute liver-specific microvasculature. LSECs line the interface between the circulatory system and hepatocytes, acting as gatekeepers of liver homeostasis and immunity. Several liver diseases are associated with the impairment of hepatic sinusoid function. In particular, although extensive studies have attested to the role of LSECs in liver pathophysiology, there are still many questions that remain unanswered regarding the mechanisms mediated by LSECs in liver homeostasis and liver diseases. This is mainly due to the complexity of liver sinusoid biology, which is hard to reproduce in hepatic models. Recently, different advanced models able to reproduce or mimic sinusoid-like structures have been described. The aim of this chapter is to summarize innovative in vitro, ex vivo, and in vivo models that enable the study of sinusoid biology and their key roles in disease development, progression, and response to drugs.

AB - Liver sinusoids are microfluidic systems formed by endothelial cells (LSECs) and other resident cells that constitute liver-specific microvasculature. LSECs line the interface between the circulatory system and hepatocytes, acting as gatekeepers of liver homeostasis and immunity. Several liver diseases are associated with the impairment of hepatic sinusoid function. In particular, although extensive studies have attested to the role of LSECs in liver pathophysiology, there are still many questions that remain unanswered regarding the mechanisms mediated by LSECs in liver homeostasis and liver diseases. This is mainly due to the complexity of liver sinusoid biology, which is hard to reproduce in hepatic models. Recently, different advanced models able to reproduce or mimic sinusoid-like structures have been described. The aim of this chapter is to summarize innovative in vitro, ex vivo, and in vivo models that enable the study of sinusoid biology and their key roles in disease development, progression, and response to drugs.

KW - Animal models

KW - Biology experimental methods

KW - Biomaterials

KW - Biomedical engineering

KW - Bioprinting

KW - Bioreactors

KW - Cell biology

KW - Cellular process

KW - Chronic liver disease

KW - Decellularization

KW - Decellularized scaffolds

KW - Extracellular matrix

KW - Hydrogels

KW - In vitro models

KW - iPSC

KW - Liver sinusoidal endothelial cells

KW - Liver-on-a-chip

KW - Materials in biotechnology

KW - Multicellular models

KW - Organoids

KW - Precision-cut liver slices

KW - Spheroids

KW - Stem cells research

KW - Tissue engineering

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M3 - Chapter

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SN - 9780323952637

SP - 135

EP - 160

BT - Sinusoidal Cells in Liver Diseases

PB - Elsevier Science Ltd.

ER -

Advanced models to study liver sinusoids

Abstract

ASJC Scopus subject areas

Keywords

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