Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.

Borbala Gerle; Anita Koroknai; György Fejer; Agnes Bakos; Ferenc Banati; Kalman Szenthe; Hans Wolf; Hans Helmut Niller; Janos Minarovits; Daniel Salamon

doi:10.1128/JVI.01396-07

Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.

Borbala Gerle
, Anita Koroknai
, György Fejer
, Agnes Bakos
, Ferenc Banati
, Kalman Szenthe
, Hans Wolf
, Hans Helmut Niller
, Janos Minarovits
, Daniel Salamon^*

^*Corresponding author for this work

School of Biomedical Sciences

Hungarian National Center for Epidemiology
Max Planck Institute of Immunobiology and Epigenetics
University of Regensburg

Research output: Contribution to journal › Article › peer-review

Abstract

We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.

Original language	English
Pages (from-to)	13242-13247
Number of pages	0
Journal	J Virol
Volume	81
Issue number	23
DOIs	https://doi.org/10.1128/JVI.01396-07
Publication status	Published - Dec 2007

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Acetylation
Cell Line
Tumor
Chromatin Immunoprecipitation
DNA
Viral
Herpesvirus 4
Human
Histones
Humans
Lymphocytes
Methylation
Molecular Sequence Data
Promoter Regions
Genetic
Protein Binding
Viral Matrix Proteins

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10.1128/JVI.01396-07

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@article{da78edaf09f3478fa67c0b379bc7f17c,

title = "Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.",

abstract = "We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.",

keywords = "Acetylation, Cell Line, Tumor, Chromatin Immunoprecipitation, DNA, Viral, Herpesvirus 4, Human, Histones, Humans, Lymphocytes, Methylation, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Viral Matrix Proteins",

author = "Borbala Gerle and Anita Koroknai and Gy{\"o}rgy Fejer and Agnes Bakos and Ferenc Banati and Kalman Szenthe and Hans Wolf and Niller, \{Hans Helmut\} and Janos Minarovits and Daniel Salamon",

year = "2007",

month = dec,

doi = "10.1128/JVI.01396-07",

language = "English",

volume = "81",

pages = "13242--13247",

journal = "J Virol",

issn = "1098-5514",

number = "23",

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TY - JOUR

T1 - Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.

AU - Gerle, Borbala

AU - Koroknai, Anita

AU - Fejer, György

AU - Bakos, Agnes

AU - Banati, Ferenc

AU - Szenthe, Kalman

AU - Wolf, Hans

AU - Niller, Hans Helmut

AU - Minarovits, Janos

AU - Salamon, Daniel

PY - 2007/12

Y1 - 2007/12

N2 - We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.

AB - We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.

KW - Acetylation

KW - Cell Line

KW - Tumor

KW - Chromatin Immunoprecipitation

KW - DNA

KW - Viral

KW - Herpesvirus 4

KW - Human

KW - Histones

KW - Humans

KW - Lymphocytes

KW - Methylation

KW - Molecular Sequence Data

KW - Promoter Regions

KW - Genetic

KW - Protein Binding

KW - Viral Matrix Proteins

U2 - 10.1128/JVI.01396-07

DO - 10.1128/JVI.01396-07

M3 - Article

SN - 1098-5514

VL - 81

SP - 13242

EP - 13247

JO - J Virol

JF - J Virol

IS - 23

ER -

Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.

Abstract

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Keywords

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