A single exposure to hyperbaric oxygen does not cause oxidative stress in isolated platelets: No effect on superoxide dismutase, catalase, or cellular ATP

Frances L. Shaw*, Richard D. Handy, Phil Bryson, J. Robert Sneyd, A. John Moody

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVES: The aim of the study was to investigate whether a single hyperbaric oxygen exposure causes oxidative stress in isolated platelets. DESIGN AND METHODS: Isolated horse platelets were exposed to 100% oxygen at 2.2 atmospheres, or 100% oxygen under normobaric conditions, or air under normobaric conditions for 90 min. RESULTS: There were no differences in platelet SOD activity between conditions, but there was a rise in SOD in all cases after 24 h (in control platelets at 24 h, SOD was 11.9 +/- 1.9 nmol/min/mg protein compared to initial background levels of 8.2 +/- 1.9 nmol/min/mg protein) (P < 0.05). Neither platelet catalase activity nor platelet GSH concentration changed over time, nor between conditions (catalase activity remained at around 12 units/mg protein, and GSH at around 1.58 nmol/mg protein). CONCLUSIONS: These data suggest that a single HBO exposure has no detrimental effect on platelet biochemistry, and does not cause overt oxidative stress in vitro.
Original languageEnglish
Pages (from-to)722-726
Number of pages5
JournalClinical Biochemistry
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 2005

ASJC Scopus subject areas

  • Clinical Biochemistry

Keywords

  • Adenosine Triphosphate
  • Animals
  • Blood Platelets
  • Catalase
  • Glutathione
  • Horses
  • Hyperbaric Oxygenation
  • In Vitro Techniques
  • L-Lactate Dehydrogenase
  • Oxidative Stress
  • Superoxide Dismutase

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