Abstract
OBJECTIVES: The aim of the study was to investigate whether a single hyperbaric oxygen exposure causes oxidative stress in isolated platelets. DESIGN AND METHODS: Isolated horse platelets were exposed to 100% oxygen at 2.2 atmospheres, or 100% oxygen under normobaric conditions, or air under normobaric conditions for 90 min. RESULTS: There were no differences in platelet SOD activity between conditions, but there was a rise in SOD in all cases after 24 h (in control platelets at 24 h, SOD was 11.9 +/- 1.9 nmol/min/mg protein compared to initial background levels of 8.2 +/- 1.9 nmol/min/mg protein) (P < 0.05). Neither platelet catalase activity nor platelet GSH concentration changed over time, nor between conditions (catalase activity remained at around 12 units/mg protein, and GSH at around 1.58 nmol/mg protein). CONCLUSIONS: These data suggest that a single HBO exposure has no detrimental effect on platelet biochemistry, and does not cause overt oxidative stress in vitro.
Original language | English |
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Pages (from-to) | 722-726 |
Number of pages | 5 |
Journal | Clinical Biochemistry |
Volume | 38 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2005 |
ASJC Scopus subject areas
- Clinical Biochemistry
Keywords
- Adenosine Triphosphate
- Animals
- Blood Platelets
- Catalase
- Glutathione
- Horses
- Hyperbaric Oxygenation
- In Vitro Techniques
- L-Lactate Dehydrogenase
- Oxidative Stress
- Superoxide Dismutase