A permeability assay for mouse intestinal organoids

Stijn Aaron den Daas; Ugo Soffientini; Shilpa Chokshi; Gautam Mehta

doi:10.1016/j.xpro.2022.101365

A permeability assay for mouse intestinal organoids

Stijn Aaron den Daas^*, Ugo Soffientini^*, Shilpa Chokshi^*, Gautam Mehta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

Original language	English
Article number	101365
Journal	STAR Protocols
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2022.101365
Publication status	Published - 17 Jun 2022
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

Keywords

Health Sciences
Model Organisms
Organoids

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10.1016/j.xpro.2022.101365

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abstract = "Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.",

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AU - den Daas, Stijn Aaron

AU - Soffientini, Ugo

AU - Chokshi, Shilpa

AU - Mehta, Gautam

PY - 2022/6/17

Y1 - 2022/6/17

N2 - Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

AB - Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

KW - Health Sciences

KW - Model Organisms

KW - Organoids

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ER -

A permeability assay for mouse intestinal organoids

Abstract

ASJC Scopus subject areas

Keywords

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