A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in
 Trichoderma reesei

Amanda Cristina Campos Antonieto; Karoline Maria Vieira Nogueira; Paula RG de; Luísa Czamanski Nora; Murilo Henrique Anzolini Cassiano; Maria Eugenia Guazzaroni; Fausto Almeida; Silva TA da; Laure Nicolas Annick Ries; Assis LJ de; Gustavo Henrique Goldman; Roberto Nascimento Silva; Rafael Silva-Rocha

doi:10.1128/msystems.00161-19

A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

Amanda Cristina Campos Antonieto
, Karoline Maria Vieira Nogueira
, Paula RG de
, Luísa Czamanski Nora
, Murilo Henrique Anzolini Cassiano
, Maria Eugenia Guazzaroni
, Fausto Almeida
, Silva TA da
, Laure Nicolas Annick Ries
, Assis LJ de
, Gustavo Henrique Goldman
, Roberto Nascimento Silva
, Rafael Silva-Rocha^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.

Original language	English
Number of pages	0
Journal	mSystems
Volume	4
Issue number	4
DOIs	https://doi.org/10.1128/msystems.00161-19
Publication status	Published - 27 Aug 2019

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A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in iTrichoderma reeseiiFinal published version, 3.04 MB

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Antonieto, A. C. C., Nogueira, K. M. V., de, P. RG., Nora, L. C., Cassiano, M. H. A., Guazzaroni, M. E., Almeida, F., da, S. TA., Ries, L. N. A., de, A. LJ., Goldman, G. H., Silva, R. N., & Silva-Rocha, R. (2019). A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei. mSystems, 4(4). https://doi.org/10.1128/msystems.00161-19

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title = "A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei",

abstract = "In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms. ",

author = "Antonieto, \{Amanda Cristina Campos\} and Nogueira, \{Karoline Maria Vieira\} and de, \{Paula RG\} and Nora, \{Lu{\'i}sa Czamanski\} and Cassiano, \{Murilo Henrique Anzolini\} and Guazzaroni, \{Maria Eugenia\} and Fausto Almeida and da, \{Silva TA\} and Ries, \{Laure Nicolas Annick\} and de, \{Assis LJ\} and Goldman, \{Gustavo Henrique\} and Silva, \{Roberto Nascimento\} and Rafael Silva-Rocha",

year = "2019",

month = aug,

day = "27",

doi = "10.1128/msystems.00161-19",

language = "English",

volume = "4",

journal = "mSystems",

issn = "2379-5077",

publisher = "American Society for Microbiology",

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T1 - A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

AU - Antonieto, Amanda Cristina Campos

AU - Nogueira, Karoline Maria Vieira

AU - de, Paula RG

AU - Nora, Luísa Czamanski

AU - Cassiano, Murilo Henrique Anzolini

AU - Guazzaroni, Maria Eugenia

AU - Almeida, Fausto

AU - da, Silva TA

AU - Ries, Laure Nicolas Annick

AU - de, Assis LJ

AU - Goldman, Gustavo Henrique

AU - Silva, Roberto Nascimento

AU - Silva-Rocha, Rafael

PY - 2019/8/27

Y1 - 2019/8/27

N2 - In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.

AB - In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.

UR - https://pearl.plymouth.ac.uk/bhs-research/305/

U2 - 10.1128/msystems.00161-19

DO - 10.1128/msystems.00161-19

M3 - Article

SN - 2379-5077

VL - 4

JO - mSystems

JF - mSystems

IS - 4

ER -

A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

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