TY - JOUR
T1 - A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in
Trichoderma reesei
AU - Antonieto, Amanda Cristina Campos
AU - Nogueira, Karoline Maria Vieira
AU - de, Paula RG
AU - Nora, Luísa Czamanski
AU - Cassiano, Murilo Henrique Anzolini
AU - Guazzaroni, Maria Eugenia
AU - Almeida, Fausto
AU - da, Silva TA
AU - Ries, Laure Nicolas Annick
AU - de, Assis LJ
AU - Goldman, Gustavo Henrique
AU - Silva, Roberto Nascimento
AU - Silva-Rocha, Rafael
PY - 2019/8/27
Y1 - 2019/8/27
N2 - In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.
AB - In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.
UR - https://pearl.plymouth.ac.uk/context/bhs-research/article/1304/viewcontent/A_20Novel_20Cys2His2_20Zinc_20Finger_20Homolog_20of_20AZF1_20Modulates_20Holocellulase_20Expression_20in_20iTrichoderma_20reeseii.pdf
U2 - 10.1128/msystems.00161-19
DO - 10.1128/msystems.00161-19
M3 - Article
SN - 2379-5077
VL - 4
JO - mSystems
JF - mSystems
IS - 4
ER -