A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES1

Declan C. Schroeder; Gaia F. Biggi; Matthew Hall; Joanne Davy; JM Martínez; Anthony J. Richardson; Gillian Malin; William H. Wilson

doi:10.1111/j.1529-8817.2005.04188.x

A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES¹

Declan C. Schroeder^*, Gaia F. Biggi, Matthew Hall, Joanne Davy, JM Martínez, Anthony J. Richardson, Gillian Malin, William H. Wilson

^*Corresponding author for this work

School of Biological and Marine Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Emiliania huxleyi(Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently,E. huxleyiis separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3′ untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.

Original language	English
Pages (from-to)	874-879
Number of pages	0
Journal	Journal of Phycology
Volume	41
Issue number	4
DOIs	https://doi.org/10.1111/j.1529-8817.2005.04188.x
Publication status	Published - Aug 2005

Access to Document

10.1111/j.1529-8817.2005.04188.x

Cite this

@article{326919f0623f444187bb7f4a06a8be3f,

title = "A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES1",

abstract = "Emiliania huxleyi(Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently,E. huxleyiis separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3′ untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.",

author = "Schroeder, {Declan C.} and Biggi, {Gaia F.} and Matthew Hall and Joanne Davy and JM Mart{\'i}nez and Richardson, {Anthony J.} and Gillian Malin and Wilson, {William H.}",

year = "2005",

month = aug,

doi = "10.1111/j.1529-8817.2005.04188.x",

language = "English",

volume = "41",

pages = "874--879",

journal = "Journal of Phycology",

issn = "0022-3646",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "4",

}

TY - JOUR

T1 - A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES1

AU - Schroeder, Declan C.

AU - Biggi, Gaia F.

AU - Hall, Matthew

AU - Davy, Joanne

AU - Martínez, JM

AU - Richardson, Anthony J.

AU - Malin, Gillian

AU - Wilson, William H.

PY - 2005/8

Y1 - 2005/8

N2 - Emiliania huxleyi(Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently,E. huxleyiis separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3′ untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.

AB - Emiliania huxleyi(Lohm.) Hay and Mohler is a ubiquitous unicellular marine alga surrounded by an elaborate covering of calcite platelets called coccoliths. It is an important primary producer involved in oceanic biogeochemistry and climate regulation. Currently,E. huxleyiis separated into five morphotypes based on morphometric, physiological, biochemical, and immunological differences. However, a genetic marker has yet to be found to characterize these morphotypes. With the use of sequence analysis and denaturing gradient gel electrophoresis, we discovered a genetic marker that correlates significantly with the separation of the most widely recognized A and B morphotypes. Furthermore, we reveal that the A morphotype is composed of a number of distinct genotypes. This marker lies within the 3′ untranslated region of a coccolith associated protein mRNA, which is implicated in regulating coccolith calcification. Consequently, we tentatively termed this marker the coccolith morphology motif.

U2 - 10.1111/j.1529-8817.2005.04188.x

DO - 10.1111/j.1529-8817.2005.04188.x

M3 - Article

SN - 0022-3646

VL - 41

SP - 874

EP - 879

JO - Journal of Phycology

JF - Journal of Phycology

IS - 4

ER -

A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES1

Abstract

Access to Document

Fingerprint

Cite this

A GENETIC MARKER TO SEPARATEEMILIANIA HUXLEYI(PRYMNESIOPHYCEAE) MORPHOTYPES¹